TA305745 TRIM2 / RNF86 (C-term) antibody

Goat Anti-TRIM2 Antibody

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0.1 mg / €325.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Goat anti Bovine, Canine, Human, Mouse, Rat TRIM2 / RNF86

Product Description for TRIM2 / RNF86

Goat anti Bovine, Canine, Human, Mouse, Rat TRIM2 / RNF86.
Properties: (C-term)
Presentation: Aff - Purified
Product is tested for Immunoprecipitation, Paraffin Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for TRIM2 / RNF86

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms KIAA0517, RING finger protein 86, Tripartite motif-containing protein 2
Presentation Aff - Purified
Reactivity Bov, Can, Hu, Ms, Rt
Applications E, IP, P, WB
Clonality Polyclonal
Host Goat
Molecular weight 81.5 kDa (Human NP_001123539.1) 84.4 kDa (Human NP_056086.2) 83.3 KDa (Rat NP_001102022.1).
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9C040
Immunogen:
Synthetic peptide from the C-Terminus of Human TRIM2 (NP_056086.2, NP_001123539.1)
AA Sequence:
DSGNHCFKVYRYLQ
GeneID:
23321
Remarks:
Percent identity with other species by BLAST analysis: Human (100%), Gorilla (100%), Gibbon (100%), Monkey (100%), Marmoset (100%), Mouse (100%), Rat (100%), Hamster (100%), Dog (100%), Bat (100%), Bovine (100%), Horse (100%), Rabbit (100%), Opossum (100%), Turkey (100%), Chicken (100%), Platypus (100%), Lizard (100%), Xenopus (100%), Pufferfish (100%), Zebrafish (100%), Pig (86%).
Property C-term
Application Peptide ELISA: 1/16000 (Detection Limit). 
Western blot: 0.5-2 µg/ml. Approx 80+85kDa bands observed in Rat Brain lysates. 
An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptide. 
Immunoprecipitation: This antibody was reported to work on Mouse protein (See Ref.1 for more details). 
Immunohistochemistry on Paraffin Sections: 6-12 µg/ml. In paraffin embedded Human Brain (Cortex) shows cytoplasm stainning in neuronal cell bodies.
Concentration 0.5 mg/ml
General Readings
  1. Thompson S, Pearson AN, Ashley MD, Jessick V, Murphy BM, Gafken P, et al. Identification of a novel Bcl-2-interacting mediator of cell death (Bim) E3 ligase, tripartite motif-containing protein 2 (TRIM2), and its role in rapid ischemic tolerance-induced neuroprotection. J Biol Chem. 2011 Jun 3;286(22):19331-9. doi: 10.1074/jbc.M110.197707. Epub 2011 Apr 8. PubMed PMID: 21478148. (Free PMC Article available, 5 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Buffer System:
Tris saline, pH~7.3
Preservatives:
0.02% Sodium Azide
Stabilizers:
0.5% BSA
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (expected):
Canine and Bovine.
Species reactivity (tested):
Human, Mouse, Rat.
Specificity
Specificity:
This antibody recognizes TRIM2 / RNF86.
Expected to recognise both reported isoforms (NP_056086.2; NP_001123539.1). 
Cross-reactivity with TRIM3 has been demonstrated.

Accessory Products

Proteins and/or Positive Controls

Positive controls for TRIM2 / RNF86 (2 products)

Catalog No. Species Pres. Purity   Source  

TRIM2 Lysate

Western Blot: TRIM2 Lysate [NBL1-17280] - Western Blot experiments. Left-Control; Right -Over-expression Lysate
  Novus Biologicals Inc.

TRIM2 overexpression lysate

TRIM2 overexpression lysate
0.1 mg / €480.00
  OriGene Technologies, Inc.
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