discontinued

SP1208P Trk antibody

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0.1 mg / €410.00

Quick Overview

Rabbit anti Human Trk

SP1208P

Product Description for Trk

Rabbit anti Human Trk.
Presentation: Purified
Product is tested for Immunoprecipitation, Western blot / Immunoblot.

Properties for Trk

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu
Applications IP, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide selected from the C-terminal catalytic domain of the human trk proto-oncogene amino acid sequence.
AA Sequence:
(Cys)-Ala-Leu-Ala-Gln-Ala-Pro-Pro-Val-Tyr-Leu-Asp-Val-Leu-Gly
Application Western Blot (1/200).
Immunoprecipitation.
Background The trk proto-oncogene encodes a 140-kilodalton, membrane-spanning protein Trk that is expressed only in neural tissues. Nerve growth factor (NGF) stimulates phosphorylation of Trk in neural cell lines and in embryonic dorsal root ganglia. Affinity cross-linking and equilibrium binding experiments indicate that Trk binds NGF specifically in cultured cells with a dissociation constant of 10(-9) molar. The identification of Trk as an NGF receptor indicates that this protein participates in the primary signal transduction mechanism of NGF. Nerve growth factor and its high-affinity receptor Trk are thought to be involved in the progression of various cancers. NGF interacts with two different low-affinity receptors that can be distinguished by affinity crosslinking. Reconstitution experiments by membrane fusion and transient transfection into heterologous cells indicate that high-affinity NGF binding requires coexpression and binding to both the low-affinity NGF receptor and Trk. These studies reveal a new growth factor receptor-mediated mechanism of cellular differentiation involving Trk and the low-affinity NGF receptor.
Concentration 0.1 mg/ml
General Readings
  1. Martin-Zanca D, Oskam R, Mitra G, Copeland T, Barbacid M. Molecular and biochemical characterization of the human trk proto-oncogene. Mol Cell Biol. 1989 Jan;9(1):24-33. PubMed PMID: 2927393. (Free PMC Article available)
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A..
Purity:
Caprylic Acid precipitation
State:
Liquid purified IgG fraction with 0.09% Sodium Azide as preservative.
Purified
Specificity
Specificity:
This antibody recognises both the Trk proto-oncogene and oncogene product (140kD and 70kD proteins respectively).
Species:
Human.

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