AP09481PU-S TrkB pTyr515 antibody

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50 µg / €230.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human, Rat TrkB

Product Description for TrkB

Rabbit anti Human, Rat TrkB.
Properties: pTyr515
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence.

Properties for TrkB

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms BDNF/NT-3 growth factors receptor, GP145-TrkB, NTRK2, Neurotrophic tyrosine kinase receptor type 2, TRKB, TrkB tyrosine kinase
Presentation Aff - Purified
Reactivity Hu, Rt
Applications ICC/IF
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q16620
Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from Human TrkB around the phosphorylation site of Tyrosine 515 (P-Q-Y-pF-G).
GeneID:
4915
Property pTyr515
Add. information Molecular Weight: 140 kDa
Application Immunofluorescence: 1/100~1/200.
Background Neurotrophins activate tyrosine kinase receptors of the Trk family (TrkA, TrkB and TrkC) and they all share a common low affinity receptor (p75NTR or p75). Although p75NTR does not appear to directly transduce NGF signals, this receptor appears to increase the responsiveness of the trk receptors and plays a role in the retrograde transport of neurotrophin signals from axon terminals to the cell body. The expression of trk gene neurotrophin receptors in mouse is tabulated by Barbacid (1994). NGF binds with high affinity and activates the TrkA tyrosine kinase receptor, which is apparently responsible for signal transduction. BDNF activates the TrkB tyrosine kinase receptor, which it shares with NT-4. NT-3 activates the TrkC tyrosine kinase receptor, but can also activate TrkA and TrkB receptors in certain cell systems. NT-4 primarily activates the TrkB tyrosine kinase receptor, which it shares with BDNF.
Concentration 1.0 mg/ml
General Readings
  1. Woronowicz A, et al. Glycobiology. 2007 Jan;17(1):10-24.
  2. Mojsilovic-Petrovic J, et al. J Neurosci. 2006 Sep 6;26(36):9250-63.
  3. Lewis MA, et al. Mol Pharmacol. 2006 Apr;69(4):1396-404.
  4. Cai D, et al. Physiol Genomics. 2006 Feb 14;24(3):191-7.
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer System:
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human, Mouse and Rat
Specificity
Specificity:
Antibody AP09481PU detects endogenous levels of TrkB only when phosphorylated at Tyrosine 515.
Gene ID 4915

Accessory Products

Proteins and/or Positive Controls

Proteins for TrkB (15 products)

Catalog No. Species Pres. Purity   Source  

TrkB (transcript variant b)

TrkB Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

TrkB (transcript variant a)

TrkB Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

TrkB (C-term DDK/His tag, transcript variant c)

TrkB Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

TrkB (C-term DDK tag, transcript variant a)

TrkB Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €411.00
  OriGene Technologies, Inc.

TrkB (transcript variant b)

TrkB Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €299.00
  OriGene Technologies, Inc.

TrkB (transcript variant b)

TrkB Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
  OriGene Technologies, Inc.

TrkB

TrkB Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

TrkB

TrkB Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

TrkB

TrkB Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

TrkB

TrkB Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

TrkB

TrkB Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

TrkB

TrkB Human
  Abnova Taiwan Corp.

TrkB

TrkB Human
  Abnova Taiwan Corp.

TrkB

TrkB Human
  Abnova Taiwan Corp.

TrkB

TrkB Human Purified
  Novus Biologicals Inc.

Positive controls for TrkB (6 products)

Catalog No. Species Pres. Purity   Source  

NTRK2 293T Cell Transient Overexpression Lysate(Denatured)

NTRK2 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

NTRK2 overexpression lysate

NTRK2 overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.

NTRK2 overexpression lysate

NTRK2 overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.

NTRK2 overexpression lysate

NTRK2 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

TrkB Lysate

Western Blot: TrkB Lysate [NBL1-13836] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for NTRK2
  Novus Biologicals Inc.

TrkB Lysate

Western Blot: TrkB Lysate [NBL1-13837] - Western Blot experiments.  Left-Control; Right -Over-expression Lysate for NTRK2.
  Novus Biologicals Inc.
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