DM3019 Troponin I (cardiac) antibody

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Mouse anti Human Troponin I (cardiac) 2D5


Product Description for Troponin I (cardiac)

Mouse anti Human Troponin I (cardiac) 2D5.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for Troponin I (cardiac)

Product Category Primary Antibodies
Quantity 0.2 mg
Presentation Purified
Reactivity Hu
Applications C, E, IP, P, WB
Clonality Monoclonal
Clone 2D5
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Purified human cardiac troponin I.
Mol. Wt. of Antigen: 21kD
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application - Immunohistochemistry 1:25-1:50.
For formalin-fixed paraffin-embedded (FFPE) tissue sections high
temperature antigen demasking is required: Deparaffinize and rehydrate tissue sections. Buffer 10 mM citrate buffer pH 6.0. Boil 10-20 mins, followed by cooling 10-20 mins
Process tissue sections in pressure cooker for 5 mins in citrate buffer followed by cooling 10-20 mins to RT. Wash tissue sections with PBS.
Note: Prolonged fixation in buffered formalin can destroy the epitope.
This product can also be used on frozen tissue sections.
- Western Blotting 1:50-1:200.
- ELISA 1:500-1:1000.
- Immunoprecipitation.
Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.4 mg/ml
General Readings
  1. Farah, C.S. et al. FASEB Journal. 9: 755-767, 1995.
  2. Katus, H.A. et al. Laboratory Medicine. 23: 311-317, 1992.
  3. Cummins, B. et al. American Heart Journ
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.
Note: Do not dilute the entire reconstituted solution at once since the diluted solution may have reduced stability. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4°C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies.
This antibody is specific for human cardiac troponin subunit I, a 21 kD protein responsible for inhibiting actomyosin Mg2+-ATPase activity. This antibody has less than 0.1% reactivity with human skeletal TNI as measured by ELISA.
Other species not tested.

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