AM00512PU-N Troponin T (cardiac) antibody

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Mouse anti Chicken, Human, Rat Troponin T (cardiac) CT-3

Product Description for Troponin T (cardiac)

Mouse anti Chicken, Human, Rat Troponin T (cardiac) CT-3.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Paraffin Sections.

Properties for Troponin T (cardiac)

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Chk, Hu, Rt
Applications ICC/IF, P
Clonality Monoclonal
Clone CT-3
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Purified rat cardiac troponin T isoform (TnT4R)
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Add. information Cellular Localization: Mostly over the I bands in myofibrils
Application Electron Microscopy.
Immunohistology (Formalin/paraffin): 1-2 µg/ml for 30 min at RT, staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Positive Control: Cardiac muscle.
Background Troponin T is a thin filament regulatory protein of striated muscle that binds to troponin C and I and to tropomyosin and is required for calcium-dependent ATPase activity of myofibrillar proteins. It is important in proper muscle function and myofibril formation. In the human heart, four cTnT isoforms are expressed in a developmentally regulated manner. This expression in the human heart is affected by heart failure.
Concentration 0.2 mg/ml
General Readings
  1. Jin and Lin, Ann. N.Y. Acad. Sci. volume 588, pp393-396 (1990)
  2. Warren and Lin J. Muscle Res Cell Motil. 14, 619-632 (1993)
Storage Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein G chromatography
Buffer System:
10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide
Liquid ascites fluid
This antibody detects Troponin T.
Human, Rat, Chicken.

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