DM3020 Troponin T (cardiac) antibody

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Mouse anti Human Troponin T (cardiac) 9B1


Product Description for Troponin T (cardiac)

Mouse anti Human Troponin T (cardiac) 9B1.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for Troponin T (cardiac)

Product Category Primary Antibodies
Quantity 0.2 mg
Presentation Purified
Reactivity Hu
Applications C, E, IP, P, WB
Clonality Monoclonal
Clone 9B1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Highly purified human cardiac troponin T.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Immunohistochemistry 1:50. Western Blotting 1:50-1:250. ELISA 1:500-1:1000. Immunoprecipitation. When used as the coating antibody in ELISAs, this antibody can be paired with affinity purified goat anti-human cardiac troponin T. This antibody can be used on formalin-fixed, paraffin embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 with LAB/Probe (Cat. No. 10-102X). When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique is strongly recommended for consistent and reproducible results. This product can also be used on frozen tissue sections. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.4 mg/ml
General Readings
  1. Farah, C.S. et al. FASEB Journal. 9: 755-767, 1995.
  2. Katrukha, A.G. et al. FEBS Letters. 315: 25-28, 1993.
  3. Katus, H.A. et al. Laboratory Medicin
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.

Aliquoting Instructions: Do not dilute the entire reconstituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
This antibody is specific for human cardiac troponin subunit T, a 31 kD protein involved in transmitting the Ca2+-binding signals responsible for contraction of cardiac muscle.

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