AM01249SU-N Trypanosoma brucei Procyclin GPEET antibody

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0.5 ml / €560.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Mouse anti Trypanosoma brucei Procyclin GPEET 9G4


Product Description for Trypanosoma brucei Procyclin GPEET

Mouse anti Trypanosoma brucei Procyclin GPEET 9G4.
Presentation: Ascites
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence.

Properties for Trypanosoma brucei Procyclin GPEET

Product Category Primary Antibodies
Quantity 0.5 ml
Presentation Ascites
Applications E, ICC/IF
Clonality Monoclonal
Clone 9G4
Host Mouse
Isotype IgG3
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Recombinant protein containing the pentapeptidyl repeat sequence of GPEET-PARP linked to the C-terminus of glutathione-S-transferase.
Application ELISA.
Background GPEET and EP procyclins are the major surface glycoproteins of Trypanosoma brucei while it lives in the midgut (procyclic form) of tsetse flies (Glossina spp.). Trypanosoma brucei is transmitted between mammals by tsetse flies and the species causes African trypanosomiasis (or sleeping sickness). The surface of Trypanosoma brucei brucei insect forms is covered by an invariant protein coat consisting of procyclins.
There are six or seven procyclin genes that encode unusual proteins with extensive tandem repeat units of glutamic acid (E) and proline (P) (referred to as EP repeats), and two genes that encode proteins with internal pentapeptide (GPEET) repeats. GPEET procyclin, is rich in Glu-Pro-Glu-Glu-Thr repeats. Although the EP forms of procyclins have been isolated and characterized by several laboratories, evidence for GPEET procyclin has largely been confined to the expression of its mRNA.
Product Citations
Purchased from Acris:
1. Capewell, P;Cren-Travaillé, C;Marchesi, F;Johnston, P;Clucas, C;Benson, RA;Gorman, TA;Calvo-Alvarez, E;Crouzols, A;Jouvion, G;Jamonneau, V;Weir, W;Stevenson, ML;O'Neill, K;Cooper, A;Swar, NK;Bucheton, B;Ngoyi, DM;Garside, P;Rotureau, B;MacLeod, A. The skin is a significant but overlooked anatomical reservoir for vector-borne African trypanosomes. Elife 2016, 5. PubMed PMID: 27653219.
General Readings
  1. Hwa KY, Acosta-Serrano A, Khoo KH, Pearson T, Englund PT. Protein glycosylation mutants of procyclic Trypanosoma brucei: defects in the asparagine-glycosylation pathway. Glycobiology. 1999 Feb;9(2):181-90. PubMed PMID: 9949195.
  2. Downey N, Donelson JE. Expression of foreign proteins in Trypanosoma congolense. Mol Biochem Parasitol. 1999 Oct 25;104(1):39-53. PubMed PMID: 10589980.
  3. Bütikofer P, Vassella E, Mehlert A, Ferguson MA, Roditi I. Characterisation and cellular localisation of a GPEET procyclin precursor in Trypanosoma brucei insect forms. Mol Biochem Parasitol. 2002 Jan;119(1):87-95. PubMed PMID: 11755189.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Lyophilized Ascitic Fluid
Restore with 0.5 ml distilled sterile water.
This antibody recognizes Trypanosoma brucei procyclin GPEET.

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