AM01250SU-N Trypanosoma brucei Procyclin phos. GPEET antibody

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Mouse anti Trypanosoma brucei Procyclin phos. GPEET 5H3


Product Description for Trypanosoma brucei Procyclin phos. GPEET

Mouse anti Trypanosoma brucei Procyclin phos. GPEET 5H3.
Presentation: Ascites
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Trypanosoma brucei Procyclin phos. GPEET

Product Category Primary Antibodies
Quantity 0.5 ml
Presentation Ascites
Applications C, E, ICC/IF, P, WB
Clonality Monoclonal
Clone 5H3
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Nour 6, an EP procyclin knockout of T. brucei procyclic trypanosomes.
Spleen cells from immunised BALB/c mice were fused with cells of the X63 Ag8.6.5.3 myeloma cell line.
Application ELISA: 1/2000.
Western Blot.
Immunofluorescence: 1/10-1/500.
This clone 5H3 has been described to work in Immunohistochemistry.
Background GPEET and EP procyclins are the major surface glycoproteins of Trypanosoma brucei while it lives in the midgut (procyclic form) of tsetse flies (Glossina spp.). Trypanosoma brucei is transmitted between mammals by tsetse flies and the species causes African trypanosomiasis (or sleeping sickness). The surface of Trypanosoma brucei brucei insect forms is covered by an invariant protein coat consisting of procyclins.
There are six or seven procyclin genes that encode unusual proteins with extensive tandem repeat units of glutamic acid (E) and proline (P) (referred to as EP repeats), and two genes that encode proteins with internal pentapeptide (GPEET) repeats. GPEET procyclin, is rich in Glu-Pro-Glu-Glu-Thr repeats. Although the EP forms of procyclins have been isolated and characterized by several laboratories, evidence for GPEET procyclin has largely been confined to the expression of its mRNA.

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Lyophilized Ascitic Fluid
Restore with 0.5ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution.
This antibody is specific for Trypanosoma brucei phosphorylated GPEET procyclin.
This antibody does not cross-react with other Trypanosome species or Leishmania.

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