NB600-615 Trypsinogen antibody

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Rabbit anti Bovine Trypsinogen


Product Description for Trypsinogen

Rabbit anti Bovine Trypsinogen.
Presentation: Ig Fraction
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Trypsinogen

Product Category Primary Antibodies
Quantity 0.5 ml
Presentation Ig Fraction
Reactivity Bov
Applications E, WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Trypsinogen from bovine pancreas.
Application ELISA, immunoprecipitation, Western Blot, In Western BLot, a 23 kDa band is seen. *Optimal dilutions should be determined by the end user., ELISA 1:2,300-1:8,400, immunoprecipitation , Western Blot 1/2000-1/10,000
Background Trypsinogen has been shown to have certain intrinsic activity. It is usually considered, however, as the inactive precursor of trypsin which may be activated by removal of a terminal hexapeptide to yield single-chain b-trypsin. Subsequent limited autolysis produces other active forms having two or more peptide chains bound by disulfide bonds. The predominant forms are a-trypsin, having two peptide chains and b-, a single chain. Different activity and thermal stability are shown by a- and b-trypsin.
General Readings Imamura T et al. Protection from pancreatitis by the zymogen granule membrane protein integral membrane-associated protein-1. J Biol Chem 277:50725-33 (2002). PubMed PMID: 12401800
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
IgG purified
Buffer System:
0.01% Sodium Azide
IgG purified
Ig Fraction
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsinogen from bovine pancreas.

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