discontinued

AM20708PU-N TUBB / TUBB5 (Loading Control) antibody

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Mouse anti Dictyostelium, Human, Mouse TUBB / TUBB5 A10

Product Description for TUBB / TUBB5

Mouse anti Dictyostelium, Human, Mouse TUBB / TUBB5 A10.
Properties: (Loading Control)
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for TUBB / TUBB5

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Tubulin beta chain, Tubulin beta-5 chain
Presentation Aff - Purified
Reactivity Dictyostelium, Hu, Ms, Saccharomyces, Tetrahymena
Applications ICC/IF, WB
Clonality Monoclonal
Clone A10
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P07437
Immunogen:
Recombinant beta-Tubulin protein from bacteria.
GeneID:
203068
Property Loading Control
Application Suitable for use in Western blot (1/1,000-1/4,000) and Immunofluorescence (1/200-1/400).
It is very useful as a Loading Control for quantitative western blotting.
Background Tubulin is a major cytoskeleton component that has five distinct forms. a and β Tubulins form heterodimers which multimerize to form a microtubule filament. There are five beta Tubulin isoforms (beta-1, beta-2, beta-3, beta-4A and beta-4B) that are expressed in mammalian tissues. Beta-1 and beta-4 are present throughout the cytosol, beta-2 is present in the nuclei and nucleoplasm, and beta-3 is a neuron-specific cytoskeletal protein.
Concentration 1.0 mg/ml
General Readings
  1. Gupta KK, et al. (2006) FEBS J; 273(23):5320-5332.
Storage Store the antibody (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Affinity Chromatography.
Buffer System:
PBS, pH 7.4 containing 0.05% Sodium Azide as preservative.
State:
Liquid purified IgG fraction.
Aff - Purified
Specificity
Specificity:
Recognize beta-Tubulin from Human, Mouse, Tetrahymena, dictyostelium, Saccharomyces.
The antibody recognizes a 50kDa band in a Western Blot analysis.
Gene ID 203068

Accessory Products

Proteins and/or Positive Controls

Proteins for TUBB / TUBB5 (5 products)

Catalog No. Species Pres. Purity   Source  

TUBB / TUBB5

TUBB / TUBB5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

TUBB / TUBB5

TUBB / TUBB5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

TUBB / TUBB5

TUBB / TUBB5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

TUBB / TUBB5

TUBB / TUBB5 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

TUBB / TUBB5

TUBB / TUBB5 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Positive controls for TUBB / TUBB5 (1 products)

Catalog No. Species Pres. Purity   Source  

beta Tubulin Lysate

Western Blot: beta Tubulin Lysate [NBL1-17435] - Western Blot experiments.  Left-Control; Right -Over-expression Lysate for TUBB.
  Novus Biologicals Inc.
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