discontinued

DM286P Tubulin alpha antibody

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Mouse anti All Species Tubulin alpha DM1A

DM286P

Product Description for Tubulin alpha

Mouse anti All Species Tubulin alpha DM1A.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for Tubulin alpha

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Microtubule Marker, alpha-Tubulin
Presentation Purified
Reactivity All Species
Applications ICC/IF, IP, P, WB
Clonality Monoclonal
Clone DM1A
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Immunogen
Immunogen:
Native Chick Brain Microtubules
Isotype control AM03095PU-N
Application Electron Microscopy(1). Immunofluorescence (1). Immunoprecipitation: Native and denatured; Use Protein G; Ab 2 micrograms/mg protein lysate. Western Blotting: 1-2 micrograms/ml for 2hrs at RT (1). Immunohistochemistry (Formalin/Paraffin): Use Ab 1-2 micrograms/ml for 30 min at RT. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues. Recommended positive control: Ls174T, MAD109 Cells, Skin, or Lung.
Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 0.2 mg/ml
General Readings
  1. Blose SH, et. al. J Cell Biol, 1984, 98:847-58.
  2. Breitlin
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.
Format
State:
Purified
Specificity
Specificity:
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kD subunits: alpha and beta. Reacts with Human, Cow, Pig, Rat, Mouse, Guinea Pig, Gerbil, Frog, Chicken, Yeast, and Fungi. Others not tested.
Cellular Localization: Cytoplasmic.

Mol. Wt. of Antigen: 57kD

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