GTX11324 Tubulin (polyglutamylated) antibody

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0.1 ml / €350.00

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Mouse anti Bovine, Human, Monkey, Mouse, Rabbit, Rat, Sea Urchin Tubulin B3

Product Description for Tubulin

Mouse anti Bovine, Human, Monkey, Mouse, Rabbit, Rat, Sea Urchin Tubulin B3.
Properties: (polyglutamylated)
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Tubulin

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Purified
Reactivity Bov, Hu, Mky, Ms, Rb, Rt, Sea Urchin
Applications E, ICC/IF, WB
Clonality Monoclonal
Clone B3
Host Mouse
Isotype IgM
Shipping to Europe only
PDF datasheet View Datasheet
Manufacturer GeneTex Inc.

Datasheet Extract

Purified sea urchin (Lytechinus pictus) sperm axonemal proteins.
Property polyglutamylated
Isotype control SM13P
Application ELISA, ICC, WB
Background The major building block of microtubules is tubulin, an intracellular cylindrical filamentous structure that is present in almost all eukaryotic cells. Except in the simplest eukaryotes, tubulin (100 kDa) exists in all cells as a heterodimer of two similar but non-identical polypeptides (55 kDa each), designated alpha and beta, that assemble into microtubules. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the carboxyl-terminal 15 residues. Both alpha and beta tubulins consist of various isotypes. In addition, both undergo post-translational modifications, including acetylation, phosphorylation detyrosination, polyglutamylation, and polyglycylation. Polyglutamylation of tubulin consists of the addition of a lateral chain of glutamyl units on the C-terminal region of tubulin polypeptides. This modification was shown to regulate the interaction between tau, one of the major neuronal microtubule associated proteins, and tubulin. Furthermore, it was shown that injection of monoclonal antibodies specific to the polyglutamylated form of tubulin into HeLa cells caused the disappearance of centrioles, an organelle known to have the most stable microtubules. The detection, localization, and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis, organellar and flagellar movement, intracellular transport, and cytoskeletal functions. Antibodies reacting specifically with the modified forms of alpha and beta tubulin isotypes serve as essential tools in the detection and study of the functional significance of these molecules.
Concentration 2.1 mg/ml
Storage Keep as concentrated solution. Store at 4
Protein A purified
Buffer System:
Preservative: 15mM Sodium Azide; Constituents: 1% BSA, 0.01M PBS. pH 7.4
Protein A purified
Cross-reacts with Human, Mouse, Rat, Cow, Chicken, Macaque Monkey, Rabbit and Sea Urchin. Not yet tested in other species.

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