NB120-11324 Tubulin (polyglutamylated) antibody

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Mouse anti Bovine, Chicken, Human, Monkey, Mouse, Rabbit, Rat Tubulin B3


Product Description for Tubulin

Mouse anti Bovine, Chicken, Human, Monkey, Mouse, Rabbit, Rat Tubulin B3.
Properties: (polyglutamylated)
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Tubulin

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Aff - Purified
Reactivity Bov, Chk, Hu, Mky, Ms, Rb, Rt, Sea urchin
Applications E, ICC/IF, WB
Clonality Monoclonal
Clone B3
Host Mouse
Isotype IgM
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Purified sea urchin (Lytechinus pictus) sperm axonemal proteins.
Property polyglutamylated
Application ELISA, Immunocytochemistry, Western Blot, ICC: Can be used with 4% paraformaldehyde-1% Triton X-100 and methanol-acetone.WB: The concentration is recommended using a cytosolic fraction of rat brain extract. Detects a band of approximately 50 kDa, possibly a doublet of alpha and beta tubulins., ELISA , Western Blot 0.5 - 1 ug/ml, Immunocytochemistry
Concentration 2.1 mg/ml
General Readings General / background references:Roach MC et al. Preparation of a monoclonal antibody specific for the class I isotype of beta-tubulin: the beta isotypes of tubulin differ in their cellular distributions within human tissues. Cell Motil Cytoskeleton 39:273-85 (1998). PubMed PMID: 9580378
Bobinnec Y et al. Centriole disassembly in vivo and its effect on centrosome structure and function in vertebrate cells. J Cell Biol 143:1575-89 (1998). PubMed PMID: 9852152
Gagnon C et al. The polyglutamylated lateral chain of alpha-tubulin plays a key role in flagellar motility. J Cell Sci 109 ( Pt 6):1545-53 (1996). PubMed PMID: 8799841
Joshi HC & Cleveland DW Diversity among tubulin subunits: toward what functional end? Cell Motil Cytoskeleton 16:159-63 (1990). PubMed PMID: 2194680
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Immunogen affinity purified
Buffer System:
15mM Sodium Azide
Immunogen affinity purified
Aff - Purified
The epitope recognized by the antibody is localized in the C-terminal region of alpha and beta tubulins (the glutamylated motif at amino acid 445-457 of alpha tubulin)., Tubulin - Polyglutamylated (B3)
Bovine, Chicken, Human, Mouse, Primate, Rabbit, Rat
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