NB120-11325 Tubulin antibody

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Mouse anti All Species Tubulin TUB-1A2

Product Description for Tubulin

Mouse anti All Species Tubulin TUB-1A2.
Presentation: Ascites
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Tubulin

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Ascites
Reactivity All Species, Various species
Applications ICC/IF, WB
Clonality Monoclonal
Clone TUB-1A2
Host Mouse
Isotype IgG3
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Synthetic peptide containing the carboxy terminal amino acids of alpha tubulin.
Application immunofluorescence, Western Blot, IF: Use at a dilution of 1/800. This concentration was determined for cultured chicken fibroblasts.WB: Use at an assay dependent dilution. Detects a band of approximately 55 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user., immunofluorescence, Western Blot
Background The tubulin family of globular proteins has several members, the most common of which are a-tubulin and ß-tubulin; proteins which make up microtubules of the cytoskeltons of probably all eukaryotic cells. Except in the simplest eukaryotes, tubulin (100 kDa) exists in all cells as a heterodimer of two similar but non-identical polypeptides (55 kDa each), designated alpha and beta. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the carboxyl-terminal 15 residues. Delta (d) and epsilon (e) tubulin have been found to localize at centrioles and may play a role in forming the mitotic spindle during mitosis, though neither is as well-studied as the a- and ß- forms.
General Readings General / background references:Kreis TE Microtubules containing detyrosinated tubulin are less dynamic. EMBO J 6:2597-606 (1987). PubMed PMID: 3315650
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer System:
15mM Sodium Azide
The reacts against tubulin's C-terminal tyrosine in immunoblotting assays and may be used for localization of this epitope in cultured cells or tissue sections.
All Species
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