NB600-567 Tubulin (acetylated) antibody

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Mouse anti Bovine, Chicken, Human, Monkey, Mouse, Plant, Porcine, Rat, Invertebrate Tubulin 6-11B-1

Product Description for Tubulin

Mouse anti Bovine, Chicken, Human, Monkey, Mouse, Plant, Porcine, Rat, Invertebrate Tubulin 6-11B-1.
Properties: (acetylated)
Presentation: Ascites
Product is tested for Dot blot, Enzyme Immunoassay, Immunoelectron Microscopy, Immunocytochemistry/Immunofluorescence, Radioimmunoassay, Western blot / Immunoblot.

Properties for Tubulin

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Ascites
Reactivity Bov, Chk, Hu, In, Mky, Ms, Pl, Por, Rt
Applications Dot, E, EM, ICC/IF, R, WB
Clonality Monoclonal
Clone 6-11B-1
Host Mouse
Isotype IgG2b
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin)
Property acetylated
Application Dot Blot, ELISA, immunohistochemistry, Western Blot, WB: The dilution was determined using bovine or rat brain preparation. Not tested in other applications. *Optimal dilutions/concentrations should be determined by the end user., ELISA, immunohistochemistry, Western Blot 1:2000, Dot Blot
Background The major building block of microtubules is tubulin, an intracellular cylindrical filamentous structure that is present in almost all eukaryotic cells. Except in the simplest eukaryotes, tubulin (100 kDa) exists in all cells as a heterodimer of two similar but non-identical polypeptides (55 kDa each), designated alpha and beta, that assemble into microtubules. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the carboxyl-terminal 15 residues. Both alpha and beta tubulins consist of various isotypes. In addition, both undergo post-translational modifications, including acetylation, phosphorylation detyrosination, polyglutamylation, and polyglycylation. Polyglutamylation of tubulin consists of the addition of a lateral chain of glutamyl units on the C-terminal region of tubulin polypeptides. This modification was shown to regulate the interaction between tau, one of the major neuronal microtubule associated proteins, and tubulin. Furthermore, it was shown that injection of monoclonal antibodies specific to the polyglutamylated form of tubulin into HeLa cells caused the disappearance of centrioles, an organelle known to have the most stable microtubules. The detection, localization, and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis, organellar and flagellar movement, intracellular transport, and cytoskeletal functions. Antibodies reacting specifically with the modified forms of alpha and beta tubulin isotypes serve as essential tools in the detection and study of the functional significance of these molecules.
General Readings
  1. LeDizet M, Piperno G. Detection of acetylated alpha-tubulin by specific antibodies. Methods Enzymol. 1991;196:264-74. PubMed PMID: 2034123.
  2. Piperno G, LeDizet M, Chang XJ. Microtubules containing acetylated alpha-tubulin in mammalian cells in culture. J Cell Biol. 1987 Feb;104(2):289-302. PubMed PMID: 2879846. (Free PMC Article available)
  3. LeDizet M, Piperno G. Identification of an acetylation site of Chlamydomonas alpha-tubulin. Proc Natl Acad Sci U S A. 1987 Aug;84(16):5720-4. PubMed PMID: 2441392. (Free PMC Article available)
  4. Piperno G, Fuller MT. Monoclonal antibodies specific for an acetylated form of alpha-tubulin recognize the antigen in cilia and flagella from a variety of organisms. J Cell Biol. 1985 Dec;101(6):2085-94. PubMed PMID: 2415535. (Free PMC Article available)
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer System:
15mM Sodium Azide
Tubulin (6-11B-1) [Lys40]
Bovine, Chicken, Hamster, Human, Mouse, Plant, Porcine, Primate, Rat, Xenopus
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