22941 Tyrosine 3-monooxygenase (TH) antibody

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0.1 ml / €520.00
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Mouse anti All Species Tyrosine 3-monooxygenase (TH) LNC1

Product Description for Tyrosine 3-monooxygenase (TH)

Mouse anti All Species Tyrosine 3-monooxygenase (TH) LNC1.
Presentation: Ascites
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Tyrosine 3-monooxygenase (TH)

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms TYH, Tyrosine 3-hydroxylase
Presentation Ascites
Reactivity All Species
Applications C, ICC/IF, P, WB
Clonality Monoclonal
Clone LNC1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer ImmunoStar Inc.

Datasheet Extract

Swiss Prot Num:
TH purified from rat PC12 cells
Application IHC Quality Control:
The antibody has wide species cross-reactivity because it recognizes an epitope in the catalytic core of the TH molecule where extensive species homology exists. TH antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat catecholamine neuron systems using indirect immunofluorescent and biotin/avidin-HRP techniques. This antibody does not cross react with phenylalanine hydroxylase or tryptophan hydroxylase.
The immunogen used to produce the TH antibody was full-length TH purified to homogeneity from PC12 cells, which are of rat origin. The exact epitope recognized by this antibody is not known but our studies indicate that following partial digestion of TH with the protease chymotrypsin the antibody recognizes the 34 kDa catalytic core of TH.
HEK293 cells were transiently transfected to express human TH protein. Protein extracts from non-transfected and transfected cells were resolved by SDS-PAGE and transferred to nitrocellulose for western blotting. The blot was probed using our monoclonal antibody to TH and then stripped and re-probed using an antibody to beta-actin. This analysis shows that only extracts derived from cells transfected to express TH contain TH protein identified by the 60 kDa immunoreactive band, while non-transfected and transfected cells both express beta-actin protein. 
Tissue: Rat striatum and substantia nigra
Perfusion Fixation: 
. Fixture: 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.
. Post Fixation: 1.5 hr. at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.
Sections: 10 μm cryostat frozen or 50 μm vibratome. 
Tissue Incubation: 18–24 hours at 2–8°C
Detection System: Use Cy3 or Bn-AV/HRP reagents at dilutions recommended by the manufacturers.
Suggested Dilution:
1/1,000 – 1/2,000 in PBS/0.3% Triton X-100 – indirect immunofluorescence.
1/4,000 – 1/8,000 in PBS/0.3% Triton X-100 Bn-Av/HRP immunohistochemistry.
Storage After reconstitution, use immediately or refrigerate at 2º-8ºC up to 2 days. For long-term storage, aliquot antibody and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles
Lyophilized ascites (100 μL)
Amphibian, Chicken, Clam, Crab, Crayfish, Crustacean, Drosophila, Fish, Fly, Frog, Gerbil, Goldfish, Guinea Pig, Hamster, Human, Leech, Lobster, Mollusca, Monkey, Mouse, Newt, Pig, Rabbit, Rat, Snail, Sparrow, Turtle, Worm, Zebra Finch, Zebrafish.
Gene ID 7054

Accessory Products

Proteins and/or Positive Controls

Proteins for Tyrosine 3-monooxygenase (TH) (2 products)

Catalog No. Species Pres. Purity   Source  

Tyrosine 3-monooxygenase (TH) (transcript variant 2)

Tyrosine 3-monooxygenase (TH) Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

Tyrosine 3-monooxygenase (TH)

Western Blot: Tyrosine Hydroxylase Protein [NBP1-42461] - Novus Biologicals antibody NB300-109 was used to detect the protein on a Western Blot.  150ng of protein were added to the Western Blot. Human Purified
  Novus Biologicals Inc.

Positive controls for Tyrosine 3-monooxygenase (TH) (1 products)

Catalog No. Species Pres. Purity   Source  

TH overexpression lysate

TH overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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