AP05192PU-N Tyrosine 3-monooxygenase (TH) pSer40 antibody

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10 Blots / €470.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human, Mouse, Rat Tyrosine 3-monooxygenase (TH)

Product Description for Tyrosine 3-monooxygenase (TH)

Rabbit anti Human, Mouse, Rat Tyrosine 3-monooxygenase (TH).
Properties: pSer40
Presentation: Aff - Purified
Product is tested for Frozen Sections, Dot blot, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Tyrosine 3-monooxygenase (TH)

Product Category Primary Antibodies
Target Category
Quantity 10 Blots
Synonyms TYH, Tyrosine 3-hydroxylase
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications C, Dot, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P07101
Immunogen:
Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser40 of rat tyrosine hydroxylase
GeneID:
7054
Property pSer40
Application Dot Blot: 1:1000.
Western Blot: 1:1000.
Immunofluorescence on frozen sections: 1:1000.
Immunohistochemistry on frozen sections: 1:1000.
Background Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982; Haycock et al., 1992; Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.
Concentration Lot specific
General Readings
  1. Dunkley PR, Bobrovskaya L, Graham ME, von Nagy-Felsobuki EI, Dickson PW. Tyrosine hydroxylase phosphorylation: regulation and consequences. J Neurochem. 2004 Dec;91(5):1025-43. PubMed PMID: 15569247.
  2. Haycock JW, Ahn NG, Cobb MH, Krebs EG. ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ. Proc Natl Acad Sci U S A. 1992 Mar 15;89(6):2365-9. PubMed PMID: 1347949. (Free PMC Article available)
  3. Haycock JW, Bennett WF, George RJ, Waymire JC. Multiple site phosphorylation of tyrosine hydroxylase. Differential regulation in situ by a 8-bromo-cAMP and acetylcholine. J Biol Chem. 1982 Nov 25;257(22):13699-703. PubMed PMID: 6128338.
  4. Jedynak JP, Ali SF, Haycock JW, Hope BT. Acute administration of cocaine regulates the phosphorylation of serine-19, -31 and -40 in tyrosine hydroxylase. J Neurochem. 2002 Jul;82(2):382-8. PubMed PMID: 12124439.
  5. Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, et al. Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology. 2001 May;24(5):561-7. PubMed PMID: 11282256.
  6. Salvatore MF, Waymire JC, Haycock JW. Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. J Neurochem. 2001 Oct;79(2):349-60. PubMed PMID: 11677263.
  7. Witkovsky P, Gabriel R, Haycock JW, Meller E. Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. J Chem Neuroanat. 2000 Jun;19(2):105-16. PubMed PMID: 10936746.
  8. Zhu M, Klimek V, Haycock JW, Ordway GA. Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Methods. 2000 Jun 30;99(1-2):37-44. PubMed PMID: 10936640.
Storage Ship on dry ice. Store (in aliquots) at -20°C to -70°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
HEPES (pH 7.5) solution containing 150 mM NaCl, 100 µg per ml BSA and 50% glycerol
Purity:
Aff - Purified
State:
Liquid purified Ig
Aff - Purified
Specificity
Specificity:
This antibody reacts to Tyrosine Hydroxylase (pSer40).
Species:
Human, Mouse, Rat.
Gene ID 7054

Accessory Products

Proteins and/or Positive Controls

Proteins for Tyrosine 3-monooxygenase (TH) (2 products)

Catalog No. Species Pres. Purity   Source  

Tyrosine 3-monooxygenase (TH) (transcript variant 2)

Tyrosine 3-monooxygenase (TH) Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

Tyrosine 3-monooxygenase (TH)

Western Blot: Tyrosine Hydroxylase Protein [NBP1-42461] - Novus Biologicals antibody NB300-109 was used to detect the protein on a Western Blot.  150ng of protein were added to the Western Blot. Human Purified
  Novus Biologicals Inc.

Positive controls for Tyrosine 3-monooxygenase (TH) (1 products)

Catalog No. Species Pres. Purity   Source  

TH overexpression lysate

TH overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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