discontinued

ARP43030_P050 UBE2M antibody

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Rabbit anti Bovine, Canine, Human, Mouse, Rat, African clawed frog UBE2M

ARP43030_P050

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Product Description for UBE2M

Rabbit anti Bovine, Canine, Human, Mouse, Rat, African clawed frog UBE2M.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for UBE2M

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms NEDD8 carrier protein, NEDD8 protein ligase, NEDD8-conjugating enzyme Ubc12, UBC12, Ubiquitin-conjugating enzyme E2 M
Presentation Aff - Purified
Reactivity African clawed frog, Bov, Can, Hu, Ms, Rt
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Europe only
PDF datasheet View Datasheet
Manufacturer AVIVA Systems Biology

Datasheet Extract

Immunogen
Swiss Prot Num:
P61081
GeneID:
9040
Add. information Rabbit Ig G is purified by peptide affinity chromatography method.
Application Suggested dilution at 1ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.
Background The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2M is a member of the E2 ubiquitin-conjugating enzyme family. It is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human UBE2M with an internal ID of P11237
General Readings Ewing,R.M., Mol. Syst. Biol. 3, 89 (2007)
Format
Buffer System:
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 ?l of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20
State:
Aff - Purified
Gene ID 9040

Accessory Products

Proteins and/or Positive Controls

Proteins for UBE2M (7 products)

Catalog No. Species Pres. Purity   Source  

UBE2M (His-tagged)

UBE2M Human Purified > 95.0% by both RP-HPLC and SDS-PAGE analyses E. coli
  OriGene Technologies GmbH

UBE2M (His-tagged)

UBE2M Human Purified > 95.0% by both RP-HPLC and SDS-PAGE analyses E. coli
  OriGene Technologies GmbH

UBE2M (His-tagged)

UBE2M Human Purified > 95.0% by both RP-HPLC and SDS-PAGE analyses E. coli
  OriGene Technologies GmbH

UBE2M (1-183, His-tag)

UBE2M Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  OriGene Technologies GmbH

UBE2M (1-183, His-tag)

UBE2M Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  OriGene Technologies GmbH

UBE2M

UBE2M Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

UBE2M

UBE2M Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for UBE2M (1 products)

Catalog No. Species Pres. Purity   Source  

UBE2M Lysate

Western Blot: UBE2M Lysate [NBL1-17540] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for UBE2M
  Novus Biologicals Inc.
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