R1564P ULP1 antibody

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0.5 mg / €440.00
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Rabbit anti Yeast ULP1

R1564P

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  • R1564P

Product Description for ULP1

Rabbit anti Yeast ULP1.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for ULP1

Product Category Primary Antibodies
Quantity 0.5 mg
Synonyms LPB11C, Ubiquitin-like-specific protease 1, YPL020C
Presentation Purified
Reactivity Ye
Applications E, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q02724
Immunogen:
Prepared from rabbit serum after repeated immunizations with recombinant yeast ULP-1 protein.
GeneID:
856087
Add. information Protein Sequence : Yeast ULP-1, 621 aa, predicted MW 72.4 kDa
1 msvevdkhrn tlqyhkknpy splfspisty rcyprvlnnp sesrrsasfs giykkrtnts
61 rfnylndrrv lsmeesmkdg sdraskagfi ggiretlwns gkylwhtfvk neprnfdgse
121 veasgnsdve srssgsrssd vpyglrenys sdtrkhkfdt stwalpnkrr riesegvgtp
181 stspisslas qksncdsdns itfsrdpfgw nkwktsaigs nsenntsdqk nsydrrqygt
241 afirkkkvak qninntklvs raqseevtyl rqifngeykv pkilkeerer qlklmdmdke
301 kdtglkksii dltekiktil iennknrlqt rnendddlvf vkekkissle rkhkdylnqk
361 lkfdrsilef ekdfkrynei lnerkkiqed lkkkkeqlak kklvpelnek dddqvqkala
421 srentqlmnr dnieitvrdf ktlaprrwln dtiieffmky iekstpntva fnsffytnls
481 ergyqgvrrw mkrkktqidk ldkiftpinl nqshwalgii dlkkktigyv dslsngpnam
541 sfailtdlqk yvmeeskhti gedfdlihld cpqqpngydc giyvcmntly gsadapldfd
601 ykdairmrrf iahliltdal k
Application This purified polyclonal antibody reacts with yeast ULP-1 by Western blot (1/500-1:2,000) and ELISA (1/4,000-1/20,000).
Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation. Expect a band approximately 72.4 kDa in size corresponding to yeast ULP-1 by western blotting in the appropriate lysate or extract.
Background ULP-1, ubiquitin-like protein-specific protease 1, initially processes Smt3 and also acts as a deconjugating enzyme for Smt3 [Saccharomyces cerevisiae (Baker's yeast)]. Covalent modification of cellular proteins by the ubiquitin-like modifier SUMO (small ubiquitin-like modifier) regulates various cellular processes, such as nuclear transport, signal transduction, stress responses and cell cycle progression. But, in contrast to ubiquination, sumoylation does not tag proteins for degradation by the 26S proteasome, but rather seems to enhance stability or modulate their subcellular compartmentalization. Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA interactions, as well as localization and stability of the target protein. Sumoylation occurs in most eukaryotic systems, and SUMO is highly conserved from yeast to humans. Where invertebrates have only a single SUMO gene termed SMT3, three members of the SUMO family have been identified in vertebrates: SUMO-1 and the close homologues SUMO-2 and SUMO-3. Three distinct steps can be distinguished in the SUMO modification pathway: 1) activation of SUMO, 2) transfer of SUMO to the conjugating enzyme, and 3) substrate modification. Since SUMO is synthesized as a precursor protein, a maturation step precedes the activation reaction. In yeast, C-terminal processing of the SUMO precursor is mediated by the processing protease Ulp1, which has an additional role in the deconjugation of SUMO-modified substrates. Mature SUMO is activated by SUMO-activating enzyme, an E1-like heterodimeric protein complex composed of Uba2 and Aos1. Ulp1 function has provided evidence that SUMO modification in yeast, as has been suspected for vertebrates, plays an important role in nucleocytoplasmic trafficking.
Concentration 5.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Li SJ, Hochstrasser M. A new protease required for cell-cycle progression in yeast. Nature. 1999 Mar 18;398(6724):246-51. PubMed PMID: 10094048.
  2. Mossessova E, Lima CD. Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast. Mol Cell. 2000 May;5(5):865-76. PubMed PMID: 10882122.
  3. Stade K, Vogel F, Schwienhorst I, Meusser B, Volkwein C, Nentwig B, et al. A lack of SUMO conjugation affects cNLS-dependent nuclear protein import in yeast. J Biol Chem. 2002 Dec 20;277(51):49554-61. Epub 2002 Oct 18. PubMed PMID: 12393908.
  4. Li SJ, Hochstrasser M. The Ulp1 SUMO isopeptidase: distinct domains required for viability, nuclear envelope localization, and substrate specificity. J Cell Biol. 2003 Mar 31;160(7):1069-81. Epub 2003 Mar 24. PubMed PMID: 12654900. (Free PMC Article available, 7 images available)
  5. Takahashi Y, Mizoi J, Toh-E A, Kikuchi Y. Yeast Ulp1, an Smt3-specific protease, associates with nucleoporins. J Biochem. 2000 Nov;128(5):723-5. PubMed PMID: 11056382.
Storage Store vial at 2-8°C prior to restoration.
After restoration, store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.
Shelf life: one year from despatch.
Format
Purification:
Multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative.
State:
Lyophilized purified Ig fraction.
Purified
Reconstitution:
Restore with 0.1 ml of deionized water (or equivalent).
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Reactivity against ULP-1 from other sources or ULP-2 has not been determined.
Gene ID 856087

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