AP31888PU-N uPA / PLAU antibody

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0.5 mg / €340.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Goat anti Human uPA / PLAU

AP31888PU-N

Product Description for uPA / PLAU

Goat anti Human uPA / PLAU.
Presentation: Aff - Purified
Product is tested for Dot blot, Enzyme Immunoassay, Frozen Sections, Paraffin Sections, Western blot / Immunoblot.

Properties for uPA / PLAU

Product Category Primary Antibodies
Target Category
Quantity 0.5 mg
Synonyms U-plasminogen activator, Urokinase, Urokinase-type plasminogen activator, Urokinase-type plasminogen activator chain B, Urokinase-type plasminogen activator long chain A, Urokinase-type plasminogen activator short chain A
Presentation Aff - Purified
Reactivity Hu
Applications C, Dot, E, P, WB
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P00749
Immunogen:
Extensively purified native HMW-tcuPA and LMW-scuPA (mixture 50/50), isolated from Human urine.
Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction, which is further subjected to absorption with immobilized total human serum proteins in order to remove non-specific antibodies.
GeneID:
5328
Application Immunoblotting Assays (Western, dot-blot).
Immunosorbent with the antibody can be used for Affinity purification of Urokinase in one step from biological fluids. 
The antibody can be used for quantitative, specific and sensitive assay for Urokinase in Human fluids (RIAELISA) in combination with the inhibiting monoclonal antibody clone PGM2001 or non-inhibiting monoclonal antibody clone PGM2005.
It is very stable and can as such be used for conjugation with peroxydase, biotine, FITC, etc. It can also be used in Immunohistochemistry on Frozen and Paraffin embedded tissue. 
Recommended Dilutions: 
Immunohistochemistry: 1/50. 
ELISA: 1/5000 (starting dilution).
Background This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Format
Purification:
Affinity Chromatogryphy: The affinity purified antibody is obtained by binding to immobilized native Human Urokinase (antigen used for immunization), followed by elution with acidic buffer, neutralization, dialysis, dispensing and lyophilization.
Buffer System:
PBS with Mannitol
State:
Lyophilized purified IgG fraction
Aff - Purified
Reconstitution:
Restore with 1 ml distilled water and add preservative if preferred.
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
The antibody binds to human HMW-scuPA (54 kDa), HMW-tsuPA (52 kDa) and LMW-scuPA (33 kDa) with high apparent affinity (Kd= 0.1 nM) completely inhibiting the ability of urokinase to activate plasminogen. Urokinase reaction with synthetic substrate S2444 is also inhibited. The antibody is highly specific for urokinase, no crossreactions were found with other serine proteases (tissue plasminogen activator, plasmin, trypsin, chymotrypsin, elastase, thrombin). Human whole plasma or plasma proteins do not interfere with its binding to urokinase.
Gene ID 5328

Accessory Products

Proteins and/or Positive Controls

Proteins for uPA / PLAU (16 products)

Catalog No. Species Pres. Purity   Source  

uPA / PLAU (transcript variant 1)

uPA / PLAU Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

uPA / PLAU (transcript variant 1)

uPA / PLAU Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
10 µg / €139.00
  OriGene Technologies, Inc.

uPA / PLAU (HMW)

uPA / PLAU Human Purified >98% pure (SDS-PAGE). 95% high molecular weight. Purified by column chromatography Urine
  Acris Antibodies GmbH

uPA / PLAU (LMW Isoform)

uPA / PLAU Human Purified >98% pure (SDS-PAGE). >98% low molecular weight. Column chromatography Urine
  Acris Antibodies GmbH

uPA / PLAU

uPA / PLAU Human Purified > 90.0% as determined by SDS-PAGE. 
Contaminents: Free of: Hepatitis B surface antigen, Hepatitis C antibody and HIV I and II.
  Acris Antibodies GmbH

uPA / PLAU

uPA / PLAU Human Purified > 90.0% as determined by SDS-PAGE. 
Contaminents: Free of: Hepatitis B surface antigen, Hepatitis C antibody and HIV I and II.
  Acris Antibodies GmbH

uPA / PLAU

uPA / PLAU Human Purified > 90.0% as determined by SDS-PAGE. 
Contaminents: Free of: Hepatitis B surface antigen, Hepatitis C antibody and HIV I and II.
  Acris Antibodies GmbH

uPA / PLAU

uPA / PLAU Human Purified > 90 % Free of Hepatitis B surface antigen, Hepatitis C antibody and HIV I and II.
Purity is greater than 90.0% as determined by SDS-PAGE.
Urine
  Acris Antibodies GmbH

uPA / PLAU

uPA / PLAU Human Purified > 90 % Free of Hepatitis B surface antigen, Hepatitis C antibody and HIV I and II.
Purity is greater than 90.0% as determined by SDS-PAGE.
Urine
  Acris Antibodies GmbH

uPA / PLAU

uPA / PLAU Human Purified > 90 % Free of Hepatitis B surface antigen, Hepatitis C antibody and HIV I and II.
Purity is greater than 90.0% as determined by SDS-PAGE.
Urine
  Acris Antibodies GmbH

uPA / PLAU

uPA / PLAU Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

uPA / PLAU

uPA / PLAU Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

uPA / PLAU

uPA / PLAU Human
  Abnova Taiwan Corp.

uPA / PLAU

uPA / PLAU Human
  Abnova Taiwan Corp.

uPA / PLAU

uPA / PLAU Human
  Abnova Taiwan Corp.

uPA / PLAU

uPA / PLAU Human
  Abnova Taiwan Corp.

Positive controls for uPA / PLAU (1 products)

Catalog No. Species Pres. Purity   Source  

Urokinase Lysate

Western Blot: Urokinase Lysate [NBL1-14486] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for PLAU
  Novus Biologicals Inc.
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