R1118BS Uricase antibody

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0.1 mg / €460.00
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Quick Overview

Goat anti Bacillus sp. Uricase


Product Description for Uricase

Goat anti Bacillus sp. Uricase.
Presentation: Biotin
Product is tested for Immunoprecipitation, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Uricase

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms EC, UOX, URIC, URO, Urate oxidase
Presentation Biotin
Reactivity Bacillus sp.
Applications E, IP, WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Uricase from Bacillus species
Application Western blot: 1/500-1/1000.
ELISA: 1/1000-1/2500;
This antibody has been assayed against 1.0 ug of uricase in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature.
Immunoprecipitation: 1:100.
Background Uricase is a peroxisomal enzyme that catalyzes the conversion of urea to allantoin. Uricase performs this action by cleaving the purine ring of uric acid rendering it much more soluble within the body for excretion. Uricase within the Bacillus species is of high importance due to its high activity and thermostability over a wide range of pH's.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation)
  2. Wu XW, Lee CC, Muzny DM, Caskey CT. Urate oxidase: primary structure and evolutionary implications. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9412-6. PubMed PMID: 2594778. (Free PMC Article available)
  3. Pfrimer P, de Moraes LM, Galdino AS, Salles LP, Reis VC, De Marco JL, et al. Cloning, purification, and partial characterization of Bacillus subtilis urate oxidase expressed in Escherichia coli. J Biomed Biotechnol. 2010;2010:674908. doi: 10.1155/2010/674908. Epub 2010 Feb 4. PubMed PMID: 20168977. (Free PMC Article available, 5 images available)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Buffer System:
0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
0.01% Sodium azide
10 mg/ml BSA (immunoglobulin and protease free)
Lyophilized purified Ig fraction
Restore with 0.1 ml of deionized water (or equivalent).
This Antibody detects Uricase [Bacillus species]. Cross reactivity against uricase from other sources is unknown. Immunoelectrophoresis gives a single precipitin arc against anti-Biotin, anti-goat serum as well as purified and partially purified uricase [Bacillus species].

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