R1118HRPS Uricase antibody

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0.1 mg / €440.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Goat anti Bacillus sp. Uricase


Product Description for Uricase

Goat anti Bacillus sp. Uricase.
Presentation: HRP
Product is tested for Immunoprecipitation, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Uricase

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms EC, UOX, URIC, URO, Urate oxidase
Presentation HRP
Reactivity Bacillus sp.
Applications E, IP, WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Uricase from Bacillus species
Application ELISA: 1/1000-1/2500.
Immunoprecipitation: 1/100.
Western blot: 1/500-1/1000.
Background Uricase is the peroxisomal enzyme responsible for the degradation of urate into allantoin. Uricase is a protein of 300 to 400 amino acids, its sequence is well conserved. It is mainly localised in the liver. In legumes, 2 forms of uricase are found: in the roots, the tetrameric form; and, in the uninfected cells of root nodules, a monomeric form, which plays an important role in nitrogen-fixation.
Caution Do NOT add Sodium azide!
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation)
  2. Wu XW, Lee CC, Muzny DM, Caskey CT. Urate oxidase: primary structure and evolutionary implications. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9412-6. PubMed PMID: 2594778. (Free PMC Article available)
  3. Pfrimer P, de Moraes LM, Galdino AS, Salles LP, Reis VC, De Marco JL, et al. Cloning, purification, and partial characterization of Bacillus subtilis urate oxidase expressed in Escherichia coli. J Biomed Biotechnol. 2010;2010:674908. doi: 10.1155/2010/674908. Epub 2010 Feb 4. PubMed PMID: 20168977. (Free PMC Article available, 5 images available)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Buffer System:
0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
0.01% Gentamicin sulfate. (Do NOT add Sodium azide!)
10 mg/mL BSA - immunoglobulin and protease free
Lyophilized purified Ig fraction
Restore with 0.1 ml of deionized water (or equivalent).
This product detects Uricase (Bacillus species). Cross reactivity against uricase from other sources is unknown.

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