R1118PS Uricase antibody

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0.1 mg / €440.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Goat anti Bacillus sp. Uricase

Product Description for Uricase

Goat anti Bacillus sp. Uricase.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for Uricase

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms EC 1.7.3.3, UOX, URIC, URO, Urate oxidase
Presentation Purified
Reactivity Bacillus sp.
Applications E, IP, WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q5WBJ3
Immunogen:
Uricase from bacillus species
GeneID:
4178593
Application Western blot: 1/500-1/1000.
ELISA: 1/1000-1/2500;
This product has been assayed against 1.0 µg of uricase [Bacillus species] in a standard sandwich ELISA using peroxidase conjugated affinity purified anti-goat IgG [H&L] (Goat) and ABTS as a substrate for 30 minutes at room temperature. 
Immunoprecipitation: 1/100.
Conjugation and most immunological methods require high titer and specificity.
Background Uricase is the peroxisomal enzyme responsible for the degradation of urate into allantoin. Uricase is a protein of 300 to 400 amino acids, its sequence is well conserved. It is mainly localised in the liver. In legumes, 2 forms of uricase are found: in the roots, the tetrameric form; and, in the uninfected cells of root nodules, a monomeric form, which plays an important role in nitrogen-fixation.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings
  1. Wu XW, Lee CC, Muzny DM, Caskey CT. Urate oxidase: primary structure and evolutionary implications. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9412-6. PubMed PMID: 2594778. (Free PMC Article available)
  2. Pfrimer P, de Moraes LM, Galdino AS, Salles LP, Reis VC, De Marco JL, et al. Cloning, purification, and partial characterization of Bacillus subtilis urate oxidase expressed in Escherichia coli. J Biomed Biotechnol. 2010;2010:674908. doi: 10.1155/2010/674908. Epub 2010 Feb 4. PubMed PMID: 20168977. (Free PMC Article available, 5 images available)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Buffer System:
0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
Preservatives:
0.01% (w/v) Sodium azide
State:
Lyophilized purified Ig fraction
Purified
Reconstitution:
Restore with 0.1 ml of deionized water (or equivalent).
Specificity
Specificity:
This product detects uricase (Bacillus species). Cross reactivity against uricase from other sources is unknown.
Immunoelectrophoresis gives a single precipitin arc against anti-goat serum as well as purified and partially purified uricase [Bacillus species].

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