AM20682PU-N VEGFR-1 / Flt-1 antibody

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0.1 mg / €390.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human VEGFR-1 / Flt-1 V12

AM20682PU-N

Product Description for VEGFR-1 / Flt-1

Mouse anti Human VEGFR-1 / Flt-1 V12.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for VEGFR-1 / Flt-1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms FLT, FLT1, FRT, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, VEGF Receptor 1, VEGFR1, Vascular endothelial growth factor receptor 1, Vascular permeability factor receptor
Presentation Purified
Reactivity Hu
Applications C, ICC/IF, WB
Clonality Monoclonal
Clone V12
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P17948
Immunogen:
Recombinant human VEGF-1 receptor
GeneID:
2321
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Western Blot: 1 - 2 μg/ml.
Immunohistochemistry on frozen sections: 2 - 4 μg/ml.
Immunocytochemistry.
Background VEGFR1, also known as FMS-related tyrosine kinase 1(FLT1). Oncogene FLT belongs to the src gene family and is related to oncogene ROS . Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. FLT is mapped to 13q12. VEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice.
Concentration 0,1 mg/ml (after reconstitution with PBS)
General Readings
  1. Imbert A, Rosnet O, Marchetto S, Ollendorff V, Birnbaum D, Pébusque MJ. Characterization of a yeast artificial chromosome from human chromosome band 13q12 containing the FLT1 and FLT3 receptor-type tyrosine kinase genes. Cytogenet Cell Genet. 1994;67(3):175-7. PubMed PMID: 7914866.
  2. Niida S, Kondo T, Hiratsuka S, Hayashi S, Amizuka N, Noda T, et al. VEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):14016-21. Epub 2005 Sep 19. PubMed PMID: 16172397. (Free PMC Article available, 6 images available)
Storage Prior to reconstitution store at -20°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography
Buffer System:
1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative
State:
Lyphilized purified Ig fraction
Purified
Reconstitution:
Restore with 1.2% sodium acetate or neutral PBS
Specificity
Specificity:
This antibody reacts to VEGFR-1.
Species:
Human.
Gene ID 2321

Accessory Products

Proteins and/or Positive Controls

Proteins for VEGFR-1 / Flt-1 (21 products)

Catalog No. Species Pres. Purity   Source  

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €788.00
  OriGene Technologies, Inc.

VEGFR-1 / Flt-1 (C-term DDK tag, transcript variant 1)

VEGFR-1 / Flt-1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €411.00
  OriGene Technologies, Inc.

VEGFR-1 / Flt-1 (His-tagged), Active Enzyme

VEGFR-1 / Flt-1 Human Purified > 70 % pure by SDS-PAGE. SF9 cells
  OriGene Technologies GmbH

VEGFR-1 / Flt-1

SDS-PAGE analysis of recombinant human soluble VEGFR-1 produced in insect cells. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 95 % pure by SDS-PAGE and visualised by silver stain Insect cells
5 µg / €200.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1

SDS-PAGE analysis of recombinant human soluble VEGFR-1 produced in insect cells. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 95 % pure by SDS-PAGE and visualised by silver stain Insect cells
20 µg / €280.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D4)

SDS-PAGE analysis of recombinant human soluble VEGFR-1D1-4 produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
5 µg / €220.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D4)

SDS-PAGE analysis of recombinant human soluble VEGFR-1D1-4 produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
20 µg / €300.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (Fc Chimera)

SDS-PAGE analysis of recombinant human soluble VEGFR-1/Fc produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
10 µg / €170.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-7) (Fc Chimera)

SDS-PAGE analysis of recombinant human soluble VEGFR-1/Fc produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
50 µg / €290.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D5)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human endogenous sFlt-1 and sFlt-1 constructs. HUVECs were stimulated with 10 ng/ml VEGF165, the soluble receptors were added with a 100X excess. Human Purified > 90 % pure by SDS-PAGE and Silver stain Insect cells
5 µg / €200.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D5)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human endogenous sFlt-1 and sFlt-1 constructs. HUVECs were stimulated with 10 ng/ml VEGF165, the soluble receptors were added with a 100X excess. Human Purified > 90 % pure by SDS-PAGE and Silver stain Insect cells
20 µg / €280.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D3)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human endogenous sFlt-1 and sFlt-1 constructs. HUVECs were
stimulated with 10 ng/ml VEGF165, the soluble receptors were added with a 100X excess. Human Purified > 90 % by SDS-PAGE and visualised by silver stain Insect cells
5 µg / €220.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D3)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human endogenous sFlt-1 and sFlt-1 constructs. HUVECs were
stimulated with 10 ng/ml VEGF165, the soluble receptors were added with a 100X excess. Human Purified > 90 % by SDS-PAGE and visualised by silver stain Insect cells
20 µg / €300.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human Purified
  Novus Biologicals Inc.

Positive controls for VEGFR-1 / Flt-1 (2 products)

Catalog No. Species Pres. Purity   Source  

FLT1 293T Cell Transient Overexpression Lysate(Denatured)

FLT1 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

VEGF Receptor 1 Lysate

Western Blot: VEGFR1 Antibody [NB100-527] - Western Blot on VEGFR1 (A) empty vector control and (B) overexpression lysates [NBL1-06699].
  Novus Biologicals Inc.
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