AP55933PU-N VEGFR-1 / Flt-1 pTyr1213 antibody

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0.1 mg / €300.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human, Mouse, Rat VEGFR-1 / Flt-1

Product Description for VEGFR-1 / Flt-1

Rabbit anti Human, Mouse, Rat VEGFR-1 / Flt-1.
Properties: pTyr1213
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for VEGFR-1 / Flt-1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms FLT, FLT1, FRT, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, VEGF Receptor 1, VEGFR1, Vascular endothelial growth factor receptor 1, Vascular permeability factor receptor
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications WB
Clonality Polyclonal
Host Rabbit
Molecular weight 90 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P17948
Immunogen:
Peptide sequence around phosphorylation site of Tyrosine 1213(V-R-Y(p)-V-N) derived from Human VEGFR1 (KLH-conjugated)
GeneID:
2321
Property pTyr1213
Application Western blot: 1:500~1:1000.
Background Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
Concentration 1.0 mg/ml
General Readings
  1. Raghu H, Nalla AK, Gondi CS, Gujrati M, Dinh DH, Rao JS. uPA and uPAR shRNA inhibit angiogenesis via enhanced secretion of SVEGFR1 independent of GM-CSF but dependent on TIMP-1 in endothelial and glioblastoma cells. Mol Oncol. 2012 Feb;6(1):33-47. doi: 10.1016/j.molonc.2011.11.008. Epub 2011 Nov 30. PubMed PMID: 22177802. (Free PMC Article available, 8 images available)
  2. Ito N, Huang K, Claesson-Welsh L. Signal transduction by VEGF receptor-1 wild type and mutant proteins. Cell Signal. 2001 Nov;13(11):849-54. PubMed PMID: 11583921.
  3. Ito N, Wernstedt C, Engström U, Claesson-Welsh L. Identification of vascular endothelial growth factor receptor-1 tyrosine phosphorylation sites and binding of SH2 domain-containing molecules. J Biol Chem. 1998 Sep 4;273(36):23410-8. PubMed PMID: 9722576.
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography using epitope-specific peptide
Buffer System:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
State:
Liquid Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human, Mouse, Rat
Specificity
Specificity:
The antibody detects endogenous level of VEGFR1 only when phosphorylated at tyrosine 1213.

Accessory Products

Proteins and/or Positive Controls

Proteins for VEGFR-1 / Flt-1 (21 products)

Catalog No. Species Pres. Purity   Source  

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €788.00
  OriGene Technologies, Inc.

VEGFR-1 / Flt-1 (C-term DDK tag, transcript variant 1)

VEGFR-1 / Flt-1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €411.00
  OriGene Technologies, Inc.

VEGFR-1 / Flt-1 (His-tagged), Active Enzyme

VEGFR-1 / Flt-1 Human Purified > 70 % pure by SDS-PAGE. SF9 cells
  OriGene Technologies GmbH

VEGFR-1 / Flt-1

SDS-PAGE analysis of recombinant human soluble VEGFR-1 produced in insect cells. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 95 % pure by SDS-PAGE and visualised by silver stain Insect cells
5 µg / €200.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1

SDS-PAGE analysis of recombinant human soluble VEGFR-1 produced in insect cells. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 95 % pure by SDS-PAGE and visualised by silver stain Insect cells
20 µg / €280.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D4)

SDS-PAGE analysis of recombinant human soluble VEGFR-1D1-4 produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
5 µg / €220.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D4)

SDS-PAGE analysis of recombinant human soluble VEGFR-1D1-4 produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
20 µg / €300.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (Fc Chimera)

SDS-PAGE analysis of recombinant human soluble VEGFR-1/Fc produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
10 µg / €170.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-7) (Fc Chimera)

SDS-PAGE analysis of recombinant human soluble VEGFR-1/Fc produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
50 µg / €290.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D5)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human endogenous sFlt-1 and sFlt-1 constructs. HUVECs were stimulated with 10 ng/ml VEGF165, the soluble receptors were added with a 100X excess. Human Purified > 90 % pure by SDS-PAGE and Silver stain Insect cells
5 µg / €200.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D5)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human endogenous sFlt-1 and sFlt-1 constructs. HUVECs were stimulated with 10 ng/ml VEGF165, the soluble receptors were added with a 100X excess. Human Purified > 90 % pure by SDS-PAGE and Silver stain Insect cells
20 µg / €280.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D3)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human endogenous sFlt-1 and sFlt-1 constructs. HUVECs were
stimulated with 10 ng/ml VEGF165, the soluble receptors were added with a 100X excess. Human Purified > 90 % by SDS-PAGE and visualised by silver stain Insect cells
5 µg / €220.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D3)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human endogenous sFlt-1 and sFlt-1 constructs. HUVECs were
stimulated with 10 ng/ml VEGF165, the soluble receptors were added with a 100X excess. Human Purified > 90 % by SDS-PAGE and visualised by silver stain Insect cells
20 µg / €300.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human Purified
  Novus Biologicals Inc.

Positive controls for VEGFR-1 / Flt-1 (2 products)

Catalog No. Species Pres. Purity   Source  

FLT1 293T Cell Transient Overexpression Lysate(Denatured)

FLT1 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

VEGF Receptor 1 Lysate

Western Blot: VEGFR1 Antibody [NB100-527] - Western Blot on VEGFR1 (A) empty vector control and (B) overexpression lysates [NBL1-06699].
  Novus Biologicals Inc.
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