DM3504 VEGFR-1 / Flt-1 antibody

See related secondary antibodies

Search for all "VEGFR-1 / Flt-1"

0.1 mg / €350.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human, Mouse VEGFR-1 / Flt-1 FLTEWC

DM3504

More Views

  • DM3504
  • DM3504

Product Description for VEGFR-1 / Flt-1

Mouse anti Human, Mouse VEGFR-1 / Flt-1 FLTEWC.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for VEGFR-1 / Flt-1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms FLT, FLT1, FRT, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, VEGF Receptor 1, VEGFR1, Vascular endothelial growth factor receptor 1, Vascular permeability factor receptor
Presentation Purified
Reactivity Hu, Ms
Applications E, IP, WB
Clonality Monoclonal
Clone FLTEWC
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P17948
Immunogen:
Recombinant Human soluble extracellular Flt-1 protein (D5) (Cat.-No DA3539X)
GeneID:
2321
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA: 1-10 µg/ml IgG1.
Western Blot: 2-5 µg/ml IgG1.
Immunoprecipitation: 1-5 µg/ml IgG1 lysate or reaction volume.
Background VEGF Receptor 1 (also known as FLT) belongs to the src gene family and shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The protein acts as a receptor for VEGF, VEGFB and PGF. An alternatively spliced form of the gene produces a soluble protein (sFlt1) which binds vascular endothelial growth factor (VEGF) with high affinity. sFlt1 has a higher affinity for VEGF indicating that it may function as an inhibitor in the VEGF response. VEGF Receptor 1 is specifically expressed in most vascular endothelial cells and peripheral blood monocytes.
VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity.
Because sFLT1 has a higher affinity for VEGF than does FLK1, it may function as an inhibitor of VEGF response.
General Readings
  1. Barleon B, Totzke F, Herzog C, Blanke S, Kremmer E, Siemeister G, et al. Mapping of the sites for ligand binding and receptor dimerization at the extracellular domain of the vascular endothelial growth factor receptor FLT-1. J Biol Chem. 1997 Apr 18;272(16):10382-8. PubMed PMID: 9099677.
  2. Roeckl W, Hecht D, Sztajer H, Waltenberger J, Yayon A, Weich HA. Differential binding characteristics and cellular inhibition by soluble VEGF receptors 1 and 2. Exp Cell Res. 1998 May 25;241(1):161-70. PubMed PMID: 9633524.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein G Chromatography
Buffer System:
PBS, pH 6.0 without preservatives or stabilizers
State:
Lyophilized purified IgG fraction from cell culture supernatant
Purified
Reconstitution:
Restore with distilled sterile water to a concentration of 0.1-1.0 mg/ml.
Species Reactivity
Species reactivity (tested):
Human and Mouse.
Specificity
Specificity:
The monoclonal antibody will detect native and denaturated VEGFR-1/Flt-1 in ELISA experiments and on the surface of different Human cell types.
The antibody will also detect Mouse Flt-1.
Gene ID 2321
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for VEGFR-1 / Flt-1 (21 products)

Catalog No. Species Pres. Purity   Source  

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

VEGFR-1 / Flt-1 (C-term DDK tag, transcript variant 1)

VEGFR-1 / Flt-1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €399.00
  OriGene Technologies, Inc.

VEGFR-1 / Flt-1 (His-tagged), Active Enzyme

VEGFR-1 / Flt-1 Human Purified > 70 % pure by SDS-PAGE. SF9 cells
10 µg / €490.00
  Acris Antibodies GmbH

VEGFR-1 / Flt-1

SDS-PAGE analysis of recombinant human soluble VEGFR-1 produced in insect cells. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 95 % pure by SDS-PAGE and visualised by silver stain Insect cells
5 µg / €190.00
  Acris Antibodies GmbH

VEGFR-1 / Flt-1

SDS-PAGE analysis of recombinant human soluble VEGFR-1 produced in insect cells. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 95 % pure by SDS-PAGE and visualised by silver stain Insect cells
20 µg / €270.00
  Acris Antibodies GmbH

VEGFR-1 / Flt-1 (D1-D4)

SDS-PAGE analysis of recombinant human soluble VEGFR-1D1-4 produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
5 µg / €200.00
  Acris Antibodies GmbH

VEGFR-1 / Flt-1 (D1-D4)

SDS-PAGE analysis of recombinant human soluble VEGFR-1D1-4 produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
20 µg / €290.00
  Acris Antibodies GmbH

VEGFR-1 / Flt-1 (Fc Chimera)

SDS-PAGE analysis of recombinant human soluble VEGFR-1/Fc produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
10 µg / €170.00
  Acris Antibodies GmbH

VEGFR-1 / Flt-1 (D1-7) (Fc Chimera)

SDS-PAGE analysis of recombinant human soluble VEGFR-1/Fc produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
50 µg / €290.00
  Acris Antibodies GmbH

VEGFR-1 / Flt-1 (D1-D5)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human endogenous sFlt-1 and sFlt-1 constructs. HUVECs were stimulated with 10 ng/ml VEGF165, the soluble receptors were added with a 100X excess. Human Purified > 90 % pure by SDS-PAGE and Silver stain Insect cells
5 µg / €190.00
  Acris Antibodies GmbH

VEGFR-1 / Flt-1 (D1-D5)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human endogenous sFlt-1 and sFlt-1 constructs. HUVECs were stimulated with 10 ng/ml VEGF165, the soluble receptors were added with a 100X excess. Human Purified > 90 % pure by SDS-PAGE and Silver stain Insect cells
20 µg / €270.00
  Acris Antibodies GmbH

VEGFR-1 / Flt-1 (D1-D3)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human endogenous sFlt-1 and sFlt-1 constructs. HUVECs were
stimulated with 10 ng/ml VEGF165, the soluble receptors were added with a 100X excess. Human Purified > 90 % by SDS-PAGE and visualised by silver stain Insect cells
5 µg / €200.00
  Acris Antibodies GmbH

VEGFR-1 / Flt-1 (D1-D3)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human endogenous sFlt-1 and sFlt-1 constructs. HUVECs were
stimulated with 10 ng/ml VEGF165, the soluble receptors were added with a 100X excess. Human Purified > 90 % by SDS-PAGE and visualised by silver stain Insect cells
20 µg / €290.00
  Acris Antibodies GmbH

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
2 µg / €300.00
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
0.1 mg / €240.00
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
10 µg / €290.00
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
0.1 mg / €1,260.00
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
5 µg / €520.00
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human Purified
10 µg / €620.00
  Novus Biologicals Inc.

Positive controls for VEGFR-1 / Flt-1 (2 products)

Catalog No. Species Pres. Purity   Source  

FLT1 293T Cell Transient Overexpression Lysate(Denatured)

FLT1 293T Cell Transient Overexpression Lysate(Denatured)
0.1 ml / €270.00
  Abnova Taiwan Corp.

VEGF Receptor 1 Lysate

Western Blot: VEGFR1 Antibody [NB100-527] - Western Blot on VEGFR1 (A) empty vector control and (B) overexpression lysates [NBL1-06699].
0.1 ml / €200.00
  Novus Biologicals Inc.
  • LinkedIn