DM3506B VEGFR-1 / Flt-1 antibody

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50 µg / €330.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Mouse anti Human, Mouse VEGFR-1 / Flt-1 FLTEWF

DM3506B

Product Description for VEGFR-1 / Flt-1

Mouse anti Human, Mouse VEGFR-1 / Flt-1 FLTEWF.
Presentation: Biotin
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for VEGFR-1 / Flt-1

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms FLT, FLT1, FRT, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, VEGF Receptor 1, VEGFR1, Vascular endothelial growth factor receptor 1, Vascular permeability factor receptor
Presentation Biotin
Reactivity Hu, Ms
Applications E, ICC/IF, IP, WB
Clonality Monoclonal
Clone FLTEWF
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P17948
Immunogen:
Recombinant Human soluble extracellular Flt-1 protein (D5) (Cat.-No DA3539)
GeneID:
2321
Isotype control SM10B (for use in human samples)
Application ELISA (1-10 µg/ml).
Western blotting (1-10 µg/ml).
Immunofluorescence.
Immunoprecipitation (2-10 µg/ml lysate or reaction volume).
Background Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA.
General Readings
  1. Barleon B, Totzke F, Herzog C, Blanke S, Kremmer E, Siemeister G, et al. Mapping of the sites for ligand binding and receptor dimerization at the extracellular domain of the vascular endothelial growth factor receptor FLT-1. J Biol Chem. 1997 Apr 18;272(16):10382-8. PubMed PMID: 9099677.
  2. Roeckl W, Hecht D, Sztajer H, Waltenberger J, Yayon A, Weich HA. Differential binding characteristics and cellular inhibition by soluble VEGF receptors 1 and 2. Exp Cell Res. 1998 May 25;241(1):161-70. PubMed PMID: 9633524.
Storage

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month 
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Label:
Biotin
Purification:
Protein G Chromatography
Buffer System:
PBS
Preservatives:
0.02% Sodium Azide
Stabilizers:
50X BSA
State:
Lyophilized purified IgG fraction
Reconstitution:
Restore with distilled sterile water to a concentration of 0.1-1.0 mg/ml.
Species Reactivity
Species reactivity (tested):
Human, Mouse.
Specificity
Specificity:
The unconjugated monoclonal antibody will recognize recombinant and naturally occurring form of VEGFR-1/Flt-1.
Gene ID 2321
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for VEGFR-1 / Flt-1 (21 products)

Catalog No. Species Pres. Purity   Source  

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

VEGFR-1 / Flt-1 (C-term DDK tag, transcript variant 1)

VEGFR-1 / Flt-1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €399.00
  OriGene Technologies, Inc.

VEGFR-1 / Flt-1 (His-tagged), Active Enzyme

VEGFR-1 / Flt-1 Human Purified > 70 % pure by SDS-PAGE. SF9 cells
  OriGene Technologies GmbH

VEGFR-1 / Flt-1

SDS-PAGE analysis of recombinant human soluble VEGFR-1 produced in insect cells. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 95 % pure by SDS-PAGE and visualised by silver stain Insect cells
5 µg / €200.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1

SDS-PAGE analysis of recombinant human soluble VEGFR-1 produced in insect cells. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 95 % pure by SDS-PAGE and visualised by silver stain Insect cells
20 µg / €280.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D4)

SDS-PAGE analysis of recombinant human soluble VEGFR-1D1-4 produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
5 µg / €220.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D4)

SDS-PAGE analysis of recombinant human soluble VEGFR-1D1-4 produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
20 µg / €300.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (Fc Chimera)

SDS-PAGE analysis of recombinant human soluble VEGFR-1/Fc produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
10 µg / €170.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-7) (Fc Chimera)

SDS-PAGE analysis of recombinant human soluble VEGFR-1/Fc produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
50 µg / €290.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D5)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human endogenous sFlt-1 and sFlt-1 constructs. HUVECs were stimulated with 10 ng/ml VEGF165, the soluble receptors were added with a 100X excess. Human Purified > 90 % pure by SDS-PAGE and Silver stain Insect cells
5 µg / €200.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D5)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human endogenous sFlt-1 and sFlt-1 constructs. HUVECs were stimulated with 10 ng/ml VEGF165, the soluble receptors were added with a 100X excess. Human Purified > 90 % pure by SDS-PAGE and Silver stain Insect cells
20 µg / €280.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D3)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human endogenous sFlt-1 and sFlt-1 constructs. HUVECs were
stimulated with 10 ng/ml VEGF165, the soluble receptors were added with a 100X excess. Human Purified > 90 % by SDS-PAGE and visualised by silver stain Insect cells
5 µg / €220.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D3)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human endogenous sFlt-1 and sFlt-1 constructs. HUVECs were
stimulated with 10 ng/ml VEGF165, the soluble receptors were added with a 100X excess. Human Purified > 90 % by SDS-PAGE and visualised by silver stain Insect cells
20 µg / €300.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human Purified
  Novus Biologicals Inc.

Positive controls for VEGFR-1 / Flt-1 (2 products)

Catalog No. Species Pres. Purity   Source  

FLT1 293T Cell Transient Overexpression Lysate(Denatured)

FLT1 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

VEGF Receptor 1 Lysate

Western Blot: VEGFR1 Antibody [NB100-527] - Western Blot on VEGFR1 (A) empty vector control and (B) overexpression lysates [NBL1-06699].
  Novus Biologicals Inc.
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