DP077-05 VEGFR-1 / Flt-1 antibody

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0.5 ml / €460.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human, Mouse, Rat VEGFR-1 / Flt-1

Product Description for VEGFR-1 / Flt-1

Rabbit anti Human, Mouse, Rat VEGFR-1 / Flt-1.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Paraffin Sections.

Properties for VEGFR-1 / Flt-1

Product Category Primary Antibodies
Target Category
Quantity 0.5 ml
Synonyms FLT, FLT1, FRT, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, VEGF Receptor 1, VEGFR1, Vascular endothelial growth factor receptor 1, Vascular permeability factor receptor
Presentation Purified
Reactivity Hu, Ms, Rt
Applications P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P17948
Immunogen:
A synthetic peptide derived from the C-terminus of the Flt-1 precursor of human origin
GeneID:
2321
Application Immunohistochemistry on Formalin-Fixed Paraffin Embedded Tissues: Use a dilution of 1/50-1/100 in an ABC method (incubation period of 30 minutes at room temperature).
Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0, prior to immunostaining.
Recommended Positive Control: Angiosarcoma.
Also useful for Western blotting.
Background VEGF Receptor 1 (also known as FLT) belongs to the src gene family and shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The protein acts as a receptor for VEGF, VEGFB and PGF. An alternatively spliced form of the gene produces a soluble protein (sFlt1) which binds vascular endothelial growth factor (VEGF) with high affinity. sFlt1 has a higher affinity for VEGF indicating that it may function as an inhibitor in the VEGF response. VEGF Receptor 1 is specifically expressed in most vascular endothelial cells and peripheral blood monocytes.
Flt-1 is a cell membrane receptor kinase. Flk-1 has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis.
General Readings
  1. Ahmed et al. Growth Factors 12: 235, 1995.
  2. Ankam-Sey et al. Oncogene 17: 115, 1998.
  3. Shibuya et al. Oncogene 5: 519, 1990.
Storage Store the antibody undiluted at 2-8°C.
Shelf life: One year from despatch.
Format
State:
Liquid purified Ig fraction with Sodium Azide as preservative.
Purified
Specificity
Specificity:
This antibody reacts with a 180 kD protein, known as Flt-1/Vascular Endothelial Growth Factor Receptor 1.
Cellular localization: cytoplasmic.
Species:
Human, Mouse and Rat.
Gene ID 2321
FocusOn and Reviews
FocusOns:

Accessory Products

Proteins and/or Positive Controls

Proteins for VEGFR-1 / Flt-1 (21 products)

Catalog No. Species Pres. Purity   Source  

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

VEGFR-1 / Flt-1 (C-term DDK tag, transcript variant 1)

VEGFR-1 / Flt-1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €399.00
  OriGene Technologies, Inc.

VEGFR-1 / Flt-1 (His-tagged), Active Enzyme

VEGFR-1 / Flt-1 Human Purified > 70 % pure by SDS-PAGE. SF9 cells
  OriGene Technologies GmbH

VEGFR-1 / Flt-1

SDS-PAGE analysis of recombinant human soluble VEGFR-1 produced in insect cells. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 95 % pure by SDS-PAGE and visualised by silver stain Insect cells
5 µg / €200.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1

SDS-PAGE analysis of recombinant human soluble VEGFR-1 produced in insect cells. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 95 % pure by SDS-PAGE and visualised by silver stain Insect cells
20 µg / €280.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D4)

SDS-PAGE analysis of recombinant human soluble VEGFR-1D1-4 produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
5 µg / €220.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D4)

SDS-PAGE analysis of recombinant human soluble VEGFR-1D1-4 produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Silver stain. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
20 µg / €300.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (Fc Chimera)

SDS-PAGE analysis of recombinant human soluble VEGFR-1/Fc produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
10 µg / €170.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-7) (Fc Chimera)

SDS-PAGE analysis of recombinant human soluble VEGFR-1/Fc produced in insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under reducing condition and stained with Coomassie blue. Human Purified > 90 % by SDS-PAGE and visualised by silver stain. Insect cells
50 µg / €290.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D5)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human endogenous sFlt-1 and sFlt-1 constructs. HUVECs were stimulated with 10 ng/ml VEGF165, the soluble receptors were added with a 100X excess. Human Purified > 90 % pure by SDS-PAGE and Silver stain Insect cells
5 µg / €200.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D5)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human endogenous sFlt-1 and sFlt-1 constructs. HUVECs were stimulated with 10 ng/ml VEGF165, the soluble receptors were added with a 100X excess. Human Purified > 90 % pure by SDS-PAGE and Silver stain Insect cells
20 µg / €280.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D3)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human endogenous sFlt-1 and sFlt-1 constructs. HUVECs were
stimulated with 10 ng/ml VEGF165, the soluble receptors were added with a 100X excess. Human Purified > 90 % by SDS-PAGE and visualised by silver stain Insect cells
5 µg / €220.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1 (D1-D3)

Inhibition of the VEGF165-induced proliferation of HUVE cells by recombinant human endogenous sFlt-1 and sFlt-1 constructs. HUVECs were
stimulated with 10 ng/ml VEGF165, the soluble receptors were added with a 100X excess. Human Purified > 90 % by SDS-PAGE and visualised by silver stain Insect cells
20 µg / €300.00
  OriGene Technologies GmbH

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human
  Abnova Taiwan Corp.

VEGFR-1 / Flt-1

VEGFR-1 / Flt-1 Human Purified
  Novus Biologicals Inc.

Positive controls for VEGFR-1 / Flt-1 (2 products)

Catalog No. Species Pres. Purity   Source  

FLT1 293T Cell Transient Overexpression Lysate(Denatured)

FLT1 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

VEGF Receptor 1 Lysate

Western Blot: VEGFR1 Antibody [NB100-527] - Western Blot on VEGFR1 (A) empty vector control and (B) overexpression lysates [NBL1-06699].
  Novus Biologicals Inc.
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