NBP1-22559 Vimentin (V9) antibody

See related secondary antibodies

Search for all "Vimentin (V9)"

Quick Overview

Mouse anti Porcine Vimentin (V9) V9

Product Description for Vimentin (V9)

Mouse anti Porcine Vimentin (V9) V9.
Presentation: Purified
Product is tested for Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot.

Properties for Vimentin (V9)

Product Category Primary Antibodies
Quantity 0.5 ml
Synonyms anti-FLJ36605 antibody, anti-OTTHUMP00000019224 antibody, anti-VIM antibody, anti-vimentin antibody
Presentation Purified
Reactivity Por
Applications IP, P, WB
Clonality Monoclonal
Clone V9
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

BALB/C mice were injected with purified vimentin from porcine eye lens.
Isotype control AM03095PU-N
Application immunohistochemistry, immunoprecipitation, Western Blot, Immunoprecipitation, Western Blot and ABC, immunoprecipitation 1:50, Western Blot 1:50-1:100, immunohistochemistry 1:25-1:50
Background Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Storage Store at 4C. Do not freeze.
protein G purified
Buffer System:
Sodium Azide
protein G purified
This reacts with the 57 kD intermediate filament protein present in the cells of mesenchymal origin. The does not show any cross-reactivity with other intermediate filament proteins. However, it shows a broad interspecies cross- reactivity.

Accessory Products

  • LinkedIn