AM20685PU-N Vinculin (VCL) antibody

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0.1 mg / €390.00
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Quick Overview

Mouse anti Chicken, Human, Mouse, Rabbit, Rat Vinculin (VCL) VIN-54

AM20685PU-N

Product Description for Vinculin (VCL)

Mouse anti Chicken, Human, Mouse, Rabbit, Rat Vinculin (VCL) VIN-54.
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Vinculin (VCL)

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Metavinculin
Presentation Purified
Reactivity Chk, Hu, Ms, Rb, Rt
Applications C, ICC/IF, WB
Clonality Monoclonal
Clone VIN-54
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P18206
Immunogen:
Human vinculin, purified from uterus
GeneID:
7414
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Western Blot: 1 - 2 μg/ml.
Immunohistochemistry on frozen sections: 2 - 4 μg/ml.
Background Vinculin is a cytoskeletal protein associated with the cytoplasmic face of both cell-cell and cell-extracellular matrix adherens-type junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane . both human and chicken embryo sequences of vinculin contain 1,066 amino acids and, furthermore, that the 2 proteins exhibit a high level of sequence identity (greater than 95%). Vinculinis mapped to 10q22.1-q23.
Concentration 0,1 mg/ml (after reconstitution with PBS)
General Readings
  1. Weller PA, Ogryzko EP, Corben EB, Zhidkova NI, Patel B, Price GJ, et al. Complete sequence of human vinculin and assignment of the gene to chromosome 10. Proc Natl Acad Sci U S A. 1990 Aug;87(15):5667-71. PubMed PMID: 2116004. (Free PMC Article available)
  2. Mulligan LM, Gardner E, Telenius H, Ponder BA. Complementary physical and genetic techniques map the vinculin (VCL) gene on chromosome 10q. Genomics. 1992 Aug;13(4):1347-9. PubMed PMID: 1505973.
Storage Prior to reconstitution store at -20°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity chromatography
Buffer System:
1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative.
State:
Lyphilized purified Ig fraction
Purified
Reconstitution:
Restore with 1.2% sodium acetate or neutral PBS.
Specificity
Specificity:
This antibody reacts to Vinculin.
Species:
Human, Mouse, Rat, Chicken.
Gene ID 7414

Accessory Products

Proteins and/or Positive Controls

Proteins for Vinculin (VCL) (3 products)

Catalog No. Species Pres. Purity   Source  

Vinculin (VCL) (transcript variant 2)

Vinculin (VCL) Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Vinculin (VCL)

Vinculin (VCL) Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Vinculin (VCL)

Vinculin (VCL) Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for Vinculin (VCL) (1 products)

Catalog No. Species Pres. Purity   Source  

Vinculin Lysate

Western Blot: Vinculin Lysate [NBL1-17706] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for VCL
  Novus Biologicals Inc.
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