AP10372PU-N Viral Protein U (58-80) antibody

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0.1 mg / €420.00
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Rabbit anti Viral Protein U

AP10372PU-N

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  • AP10372PU-N

Product Description for Viral Protein U

Rabbit anti Viral Protein U.
Properties: (58-80)
Presentation: Aff - Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Viral Protein U

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms U ORF protein, VPU
Presentation Aff - Purified
Applications C, E, ICC/IF, IP, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide corresponding to amino acids 58-80 of Viral Protein U. This peptide was selected from the unique region of the protein.
AA Sequence:
EGD QEE LSA LME MGH HAP WNV ND
Remarks:
This peptide was post-synthetically modified to achieve highest antigenicity and coupled to KLH using heterobifunctional cross linker.
Property 58-80
Control peptide AP10372CP-N
Application ELISA (1/50000).
Western Blot (1/1000).
Immunoprecipitation: 1/250.
Immunohistochemistry (1/250).
Immunofluorescence (1/250).
Western blot or Immunofluorescence have been tested using HEK293 cells transfected with HIV-1 Vpu cDNA at 1/200 and 1/500 dilutions.
Background Retroviruses have several characteristic structural and catalytic proteins, one such auxiliary protein is a viral protein U (Vpu) which enhances virion release from human cells and also involved in the degradation of CD4, the cellular surface receptor of HIV-1. The Vpu has no homolog in less pathogenic HIV-2 virus. Vpu is an 81 amino acid class I membrane integral protein that is unique to human and simian immunodeficiency virus isolated from Chimpanzee and few other monkey species. The 16kDa protein Vpu protein consist of an N-terminal hydrophobic membrane anchor of 27 amino acids and a charged C-terminal hydrophilic domain of 54 amino acids that extents to the cytoplasm. The cytoplasmic domain has a conserved dual serine phosphorylation site (S52 GXXand S56 motif) that is phosphorylated by casein kinase II (1).
Vpu is involved in viral replication an degradation of its cellular receptor CD4 and enhancement of viral particle release from macrophages and primary lymphocytes. The degradation of CD4 receptor is achieved by hijacking of protein degradation machinery of the host cells that involves ubiquitin ligases that ensures the selection of proteins to be degraded. Vpu binds to CD4 and simultaneously recruits the βTrCP subunit of the SCFβTrCP ubiquitin ligase complex through its constitutively phosphorylated DS52GXXS56 motif. In this process, Vpu was found to escape degradation, while inhibiting the degradation of βTrCP natural targets such as β-catenin and IκBα (2). Interestingly, the Vpu activity was not observed in simian cells probably due to its ability to counter act host cell restriction factor specific for human cells and may depend on Vpu binding to host channel TASK-1 protein (3). Vpu is degraded in cells arrested in early mitosis by nacodazole, the degradation process require phosphorylation of the serine 61 residue adjacent to the bTrCP-binding motif (3). Vpu has all the characteristics of signal peptide sequences (hydrophobic N-terminal and a hydrophilic C-terminal tail) when cleaved by signal peptidases stays with lipids of the signal peptidase complex, after further processing the N-0terminal region is released into cytosol where it interacts with calmodulin and preprolactin.
Concentration 0.65 mg/ml (lot. sepcific)
Product Citations
Purchased from Acris:
  1. Leznicki P, High S. SGTA antagonizes BAG6-mediated protein triage. Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19214-9. doi: 10.1073/pnas.1209997109. Epub 2012 Nov 5. PubMed PMID: 23129660. (Free PMC Article available, 4 images available)
  2. Park SY, Waheed AA, Zhang ZR, Freed EO, Bonifacino JS. HIV-1 Vpu accessory protein induces caspase-mediated cleavage of IRF3 transcription factor. J Biol Chem. 2014 Dec 19;289(51):35102-10. doi: 10.1074/jbc.M114.597062. Epub 2014 Oct 28. PubMed PMID: 25352594. (Free PMC Article available)
General Readings
  1. Julie Binette., Mathieu Dube., Johanne Mercier., et. al., Requirement for the selective degradation of CD4 receptor molecule by the human immunodeficiency virus type 1 Vpu protein in endoplasmic reticulum. Retrovirology, 2007. 4. 75.
  2. Estrabaud E, Le Rouzic E, Lopez-Vergès S, Morel M, Belaïdouni N, Benarous R, et al. Regulated degradation of the HIV-1 Vpu protein through a betaTrCP-independent pathway limits the release of viral particles. PLoS Pathog. 2007 Jul 27;3(7):e104. PubMed PMID: 17676996. (Free PMC Article available, 6 images available)
  3. Hsu K, Seharaseyon J, Dong P, Bour S, Marbán E. Mutual functional destruction of HIV-1 Vpu and host TASK-1 channel. Mol Cell. 2004 Apr 23;14(2):259-67. PubMed PMID: 15099524.
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Antigen-Sepharose Affinity Chromatography
Buffer System:
Stabilization buffer with 0.02% Sodium Azide as preservative
State:
Liquid purified Ig fraction
Aff - Purified
Specificity
Specificity:
Reacts with HIV1.
Species:
HIV-1

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