NB600-950 VP16 tag antibody

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0.1 ml / €400.00
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Quick Overview

Rabbit anti VP16 tag


Product Description for VP16 tag

Rabbit anti VP16 tag.
Presentation: Aff - Purified
Product is tested for Immunoprecipitation, Western blot / Immunoblot.

Properties for VP16 tag

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Aff - Purified
Applications IP, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Synthetic peptide corresponding to amino acids 464-476 of the herpes simplex virus VP16 protein, conjugated to KLH via an N-terminal added cysteine.
Application immunoprecipitation, Western Blot, Immunoprecipitation and Western Blot: Optimal working dilutions should be determined by investigator., immunoprecipitation 10 ug/ml, Western Blot 1/1000
Background VP16 from herpes simple virus (HSV) is a strong transcriptional activator of immediate early viral genes.1 Due to its lack of DNA binding activity, VP16 is recruited to DNA via interaction with DNA binding proteins.2 Ptashne et al. showed that VP16 can function as a potent transcriptional activator in mammalian cells when fused to the DNA binding domain of the yeast transcriptional activator GAL4.3 These properties were taken into consideration in the development of a mammalian version of the Yeast Two-Hybrid System.4-6 The Yeast Two-Hybrid System is a powerful method for characterizing and screening for protein-protein interactions. In the original version of the system, GAL4 DNA binding domain is fused to protein X, and GAL4 activation domain is fused to protein Y. Neither hybrid is capable of activating transcription individually. Upon interaction between proteins X and Y, the DNA binding and activator domains are brought in close proximity. As a result, the transcriptional activity of GAL4 is reconstituted at the promoter, and monitored with a reporter gene.4 Additional two-hybrid systems were developed using different DNA binding and activation domains as well as different reporter genes.5-9 The VP16 activator is used in yeast and mammalian systems. In yeast, its DNA interaction partner is typically LexA.10 In mammalian systems, interaction between two proteins is detected through recruitment of VP16 and GAL4 DNA binding domain to GAL4 operators present in the reporter gene.7,9-11
Concentration 0.5 mg/ml
General Readings
  1. Pellett, P.E., et al., Proc. Natl. Acad. Sci. USA, 82, 5870-5874 (1985).
  2. Flint, J., and Shenk, T., Annu. Rev. Genet., 31, 177-212 (1997).
  3. Sadowski, I., et al., Nature, 335, 563-564 (1988).
  4. Fields, S., and Song, O., Nature, 340, 245-246 (1989).
  5. Fields, S., and Sternglanz, R., Trends Genet., 10, 286-292 (1994).
  6. Marti, F., et al., Proc. Natl. Acad. Sci. USA , 95, 11810-11815 (1998).
  7. Zhang, D., et al., Endocrinol., 143, 62-73 (2002).
  8. Leonhardt, S.A., et al., Molec. Endocrinol., 12, 1914-1930 (1998).
  9. Teyssier, C., et al., J. Biol. Chem., 276, 36361-36369 (2001).
  10. Hollenberg, S.M., et al., Mol. Cell. Biol., 15, 3813-3822 (1995).
  11. Fagan, R., et al., Cell, 78, 799-811 (1994)
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Immunogen affinity purified
Buffer System:
15mM Sodium Azide
Immunogen affinity purified
Aff - Purified
Recognizes VP16 fusion protein.

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