discontinued

AM01311FC-N WC14 antibody

See related secondary antibodies

Search for all "WC14"

0.1 mg / €560.00

Quick Overview

Mouse anti Bovine WC14 BT3/8.12

AM01311FC-N

Product Description for WC14

Mouse anti Bovine WC14 BT3/8.12.
Presentation: FITC
Product is tested for Flow Cytometry.

Properties for WC14

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation FITC
Reactivity Bov
Applications F
Clonality Monoclonal
Clone BT3/8.12
Host Mouse
Isotype IgG3
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Theileria parva infected bovine lymphoblastoid cell line. Spleen cells from immunised BALB/c mice were fused with cells of the P3-NS1/1-Ag4-1 myeloma cell line.
Isotype control AM03097FC-N
Add. information Clone BT3/8.12 is reported to immunoprecipitate two polypeptide chains of 150kDa and 158kDa from activated T cells, under reducing conditions (ref.1)
Application Flow cytometry (Neat - 1/5): use 10 µl of the suggested working dilution to label 1x10e6 cells in 100 µl.
Concentration 0.1 mg/ml
General Readings
  1. Naessens, J. et al. (1985) De novo expression of T cell markers on Theileria parva- transformed lymphoblasts in cattle. J. Immunol. 135: 4183 - 4188.
  2. Muiya P, Logan-Henfrey L, Naessens J. Expression of antigens on haemopoietic progenitor cells in bovine bone marrow. Vet Immunol Immunopathol. 1993 Nov;39(1-3):237-48. PubMed PMID: 8310648.
  3. Naessens J, Nthale JM, Muiya P. Biochemical analysis of preliminary clusters in the non-lineage panel. Vet Immunol Immunopathol. 1996 Aug;52(4):347-56. PubMed PMID: 8896224.
  4. Howard, C.J. et al. (1996) Ruminant cluster WC14. Vet. Immunol. Immunopathol. 52: 261 - 262.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Format
Label:
Fluorescein Isothiocyanate Isomer 1
FITC
Purification:
Affinity chromatography on Protein G
Buffer System:
PBS, pH 7.4, containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
State:
Liquid purified IgG
Specificity
Specificity:
This antibody recognises the WC14 cell surface antigen, which is expressed on monocytes and granulocytes, and a subset of activated T cells.
Species:
Bovine.

Accessory Products

  • LinkedIn