AM01311PU-N WC14 antibody

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0.25 mg / €480.00

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Mouse anti Bovine WC14 BT3/8.12


Product Description for WC14

Mouse anti Bovine WC14 BT3/8.12.
Presentation: Purified
Product is tested for Immunoprecipitation, Flow Cytometry.

Properties for WC14

Product Category Primary Antibodies
Quantity 0.25 mg
Presentation Purified
Reactivity Bov
Applications F, IP
Clonality Monoclonal
Clone BT3/8.12
Host Mouse
Isotype IgG3
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Theileria parva infected bovine lymphoblastoid cell line. Spleen cells from immunised BALB/c mice were fused with cells of the P3-NS1/1-Ag4-1 myeloma cell line.
Application Immunoprecipitation.
Flow Cytometry: 1/50 - 1/100; Use 10µl of the suggested working dilution to label 1x10e6 cells in 100 µl.
Background WC14 is expressed on monocytes and granulocytes, and a subset of activated T cells. Clone BT3/8.12 is reported to immunoprecipitate two polypeptide chains of 150kDa and 158kDa from activated T cells, under reducing conditions.
Concentration 1.0 mg/ml
General Readings
  1. Naessens, J. et al. (1985) De novo expression of T cell markers on Theileria parva- transformed lymphoblasts in cattle. J. Immunol. 135: 4183 - 4188.
  2. Muiya P, Logan-Henfrey L, Naessens J. Expression of antigens on haemopoietic progenitor cells in bovine bone marrow. Vet Immunol Immunopathol. 1993 Nov;39(1-3):237-48. PubMed PMID: 8310648.
  3. Naessens J, Nthale JM, Muiya P. Biochemical analysis of preliminary clusters in the non-lineage panel. Vet Immunol Immunopathol. 1996 Aug;52(4):347-56. PubMed PMID: 8896224.
  4. Howard, C.J. et al. (1996) Ruminant cluster WC14. Vet. Immunol. Immunopathol. 52: 261 - 262.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity chromatography on Protein G
Buffer System:
PBS, pH 7.4 containing 0.09% Sodium Azide
Liquid purified Ig
This antibody recognises the bovine WC14 cell surface antigen.

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