TA319950 WIPI-1 (C-term) antibody

Rabbit Polyclonal WIPI1 Antibody

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0.1 mg / €325.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Rabbit anti Human, Mouse, Rat WIPI-1

TA319950

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  • TA319950

Product Description for WIPI-1

Rabbit anti Human, Mouse, Rat WIPI-1.
Properties: (C-term)
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for WIPI-1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Atg18 protein homolog, WD repeat domain phosphoinositide-interacting protein 1, WD40 repeat protein interacting with phosphoinositides of 49 kDa, WIPI1, WIPI49
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications E, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Molecular weight 49 kDa
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
Q5MNZ9
Immunogen:
17 amino acid synthetic peptide near the carboxy terminus of Human WIPI1 (AP55473CP-N)
GeneID:
55062
Property C-term
Add. information Blocking peptide available: AP55473CP-N
Application Western blot: 1-2 μg/ml.
Positive Control: Rat colon tissue lysate. 
Immunofluorescence: Start at 20 μg/ml.
Background WIPI1 (WD repeat domain, phosphoinositide interacting-1), also known as WIPI1, ATG18 or WIPI49, is thought to play a role in autophagy and may regulate protein trafficking in certain recycling pathways. It contains three WD repeats and has a 7-bladed propeller structure with a conserved motif that facilitates its interaction with other proteins. WIPI1 localizes to cytoplasmic vesicles, endosomes, clathrin-coated vesicles and the trans-Golgi network. It is ubiquitously expressed with highest expression in heart, testis, placenta, pancreas and skeletal muscle. WIPI1 is upregulated in a variety of tumors, suggesting a role in carcinogenesis.
Concentration 1.0 mg/ml
General Readings
  1. Proikas-Cezanne T, Robenek H. Freeze-fracture replica immunolabelling reveals human WIPI-1 and WIPI-2 as membrane proteins of autophagosomes. J Cell Mol Med. 2011 Sep;15(9):2007-10. doi: 10.1111/j.1582-4934.2011.01339.x. PubMed PMID: 21564513.
  2. Jeffries TR, Dove SK, Michell RH, Parker PJ. PtdIns-specific MPR pathway association of a novel WD40 repeat protein, WIPI49. Mol Biol Cell. 2004 Jun;15(6):2652-63. Epub 2004 Mar 12. PubMed PMID: 15020712. (Free PMC Article available, 10 images available)
  3. Proikas-Cezanne T, Waddell S, Gaugel A, Frickey T, Lupas A, Nordheim A. WIPI-1alpha (WIPI49), a member of the novel 7-bladed WIPI protein family, is aberrantly expressed in human cancer and is linked to starvation-induced autophagy. Oncogene. 2004 Dec 16;23(58):9314-25. PubMed PMID: 15602573.
  4. Proikas-Cezanne T, Ruckerbauer S, Stierhof YD, Berg C, Nordheim A. Human WIPI-1 puncta-formation: a novel assay to assess mammalian autophagy. FEBS Lett. 2007 Jul 24;581(18):3396-404. Epub 2007 Jun 27. PubMed PMID: 17618624.
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Purification:
Affinity chromatography purified via peptide column
Buffer System:
PBS containing 0.02% Sodium Azide as preservative
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human, Mouse, Rat.
Specificity
Specificity:
At least two isoforms of WIPI1 are known to exist, this antibody will detect both isoforms.

Accessory Products

Proteins and/or Positive Controls

Proteins for WIPI-1 (4 products)

Catalog No. Species Pres. Purity   Source  

WIPI-1

WIPI-1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

WIPI-1

WIPI-1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

WIPI-1

WIPI-1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

WIPI-1

WIPI-1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

Positive controls for WIPI-1 (2 products)

Catalog No. Species Pres. Purity   Source  

WIPI1 Lysate

Western Blot: WIPI1 Lysate [NBL1-17853] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for WIPI1
  Novus Biologicals Inc.

WIPI1 overexpression lysate

WIPI1 overexpression lysate
0.1 mg / €280.00
  OriGene Technologies, Inc.
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