AP00591PU-N WNT3A antibody

See related secondary antibodies

Search for all "WNT3A"

0.1 mg / €340.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human WNT3A

Product Description for WNT3A

Rabbit anti Human WNT3A.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot, Paraffin Sections.

Properties for WNT3A

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Protein Wnt-3a
Presentation Aff - Purified
Reactivity Hu
Applications E, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P56704
Immunogen:
E.Coli derived recombinant Human WNT-3a
GeneID:
89780
Add. information Centrifuge vial prior to opening.
Application Sandwich ELISA: To detect Human WNT-3a by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml is required. This affinity purified polyclonal antibody, in conjunction with Biotin conjugated Polyclonal Anti-Human WNT-3a (AP00591BT-N or AP00591BT-S) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human WNT-3a.
Western Blot: To detect Human WNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Immunohistochemistry: 0.125-0.25 µg/ml with an overnight incubation at 4°C.
This antibody stained Formalin-Fixed, Paraffin-Embedded sections of Human breast invasive ductal carcinoma. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommneded. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography
Buffer System:
PBS, pH 7.2 without preservatives
Endotoxin level:
< 0.1 ng/µg of Protein
State:
Lyophilized (sterile filtered) purified Ig fraction
Aff - Purified
Reconstitution:
Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Specificity
Specificity:
This antibody detects Human WNT-3a. Other species not tested.
Gene ID 89780

Accessory Products

Proteins and/or Positive Controls

Proteins for WNT3A (5 products)

Catalog No. Species Pres. Purity   Source  

WNT3A (19-352, )

WNT3A Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  OriGene Technologies GmbH

WNT3A (19-352, )

WNT3A Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  OriGene Technologies GmbH

WNT3A

WNT3A Human Purified
  Abnova Taiwan Corp.

WNT3A

WNT3A Human Purified
  Abnova Taiwan Corp.

WNT3A

WNT3A Human Purified
  Abnova Taiwan Corp.

Positive controls for WNT3A (3 products)

Catalog No. Species Pres. Purity   Source  

WNT3A 293T Cell Transient Overexpression Lysate(Denatured)

WNT3A 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

Wnt3a Lysate

Western Blot: Wnt3a Lysate [NBL1-17870] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for WNT3A
  Novus Biologicals Inc.

WNT3A overexpression lysate

WNT3A overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
  • LinkedIn