discontinued

SP7355A WNVE (73-86) antibody

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0.1 mg / €400.00

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Rabbit anti WNVE

SP7355A

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  • SP7355A

Product Description for WNVE

Rabbit anti WNVE.
Properties: (73-86)
Presentation: Azide Free
Product is tested for Paraffin Sections, Western blot / Immunoblot.

Properties for WNVE

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Azide Free
Applications P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide corresponding to 73-86 of West Nile virus envelope glycoprotein E
AA Sequence:
CPTMGEAHNEKRAD
Property 73-86
Add. information Immunohistochemistry Picture below: The antigen retrieval method used to obtain staining: Ventana Protease 2 for three minutes at room temperature with a Parafilm coverslip.
SP7355P was used at an initial concentration of 1 µg/ml (using an autostainer which keeps residual fluid on the slides during every step, so the final concentration is approximately 2.5 times more dilute). Image and protocol used to generate this image are courtesy of Angela Ellis, University of Georgia College of Veterinary Medicine.
Application Western blot: 1-3 µg/ml. 
Immunohistochemistry on Paraffin Sections: 1 µg/ml. 
Recommended Positive Control: West Nile virus infected cell line.
Background West Nile (WN), the most widespread among flaviviruses, was first isolated from the serum of a febrile woman in 1937 in the West Nile district of Uganda. West Nile virus was first detected in North America in 1999 and has subsequently spread throughout the United States and Canada and into Mexico and the Caribbean. In Africa, southern Europe, western Asia, and the United States, WNV has been isolated from mosquitoes of more than 40 species. In the United States, Canada, and Israel, WNV is responsible for significant avian mortality.
Concentration 0.5 mg/ml
General Readings
  1. Smithburn JS, Hughes TP, Burke AW, Paul JH. A neurotropic virus isolated from the blood of a native of Uganda. Am. J. Trop. Med. Hyg. 20: 471–492 (1970).
  2. Hayes, C.G. West Nile fever. In The arboviruses: epidemiology and ecology. T.P. Monath, editor. CRC Press. Boca Raton, Florida, USA. 59–88 (1989).
  3. Yamshchikov VF, Wengler G, Perelygin AA, Brinton MA, Compans RW. An infectious clone of the West Nile flavivirus. Virology. 2001 Mar 15;281(2):294-304. PubMed PMID: 11277701.
  4. Laboratory-acquired West Nile virus infections — United States, 2002. Morb. Mortal. Wkly Rep. 51:1133–1135 (2002).
  5. L. Hannah Gould and Erol Fikrig. West Nile virus: a growing concern? J Clin Invest. 113(8): 1102–1107 (2004).
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Purification:
Protein G Chromatography
Buffer System:
PBS, filter sterilized
Preservatives:
None
State:
Liquid purified Ig fraction, Azide Free
Azide Free
Specificity
Specificity:
This antibody detetcs WNVE.

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