discontinued

AP15857PU-N WT1 / Wilms tumor protein antibody

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Rabbit anti Canine, Human, Mouse, Porcine, Rabbit, Rat WT1 / Wilms tumor protein

Product Description for WT1 / Wilms tumor protein

Rabbit anti Canine, Human, Mouse, Porcine, Rabbit, Rat WT1 / Wilms tumor protein.
Presentation: Purified
Product is tested for Immunoprecipitation, Paraffin Sections, Western blot / Immunoblot.

Properties for WT1 / Wilms tumor protein

Product Category Primary Antibodies
Target Category
Quantity 1 ml
Synonyms WT33
Presentation Purified
Reactivity Can, Hu, Ms, Por, Rb, Rt
Applications IP, P, WB
Clonality Polyclonal
Host Rabbit
Molecular weight 47-55 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P19544
Immunogen:
Synthetic peptide derived from the C-terminus of Human Wilm's Tumor protein.
GeneID:
7490
Application Western Blot.
Immunoprecipitation.
Immunohistochemistry on Paraffin Sections: 1/100 for 10 min at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Positive Control: Wilm's Tumor.
Background Wilm's Tumor (WT), a sporadic and familial childhood kidney tumor, is genetically heterogeneous. Wilm's tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed infetal kidney and mesothelioma, and its expression has been suggested as a marker for Wilm's tumor and mesothelioma.
Concentration 0.69 mg/ml (Lot specific)
Product Citations
Purchased from Acris:
1. Yates LL, Papakrivopoulou J, Long DA, Goggolidou P, Connolly JO, Woolf AS, et al. The planar cell polarity gene Vangl2 is required for mammalian kidney-branching morphogenesis and glomerular maturation. Hum Mol Genet. 2010 Dec 1;19(23):4663-76. doi: 10.1093/hmg/ddq397. Epub 2010 Sep 14. PubMed PMID: 20843830
2. Vasilopoulou, E;Kolatsi-Joannou, M;Lindenmeyer, MT;White, KE;Robson, MG;Cohen, CD;Sebire, NJ;Riley, PR;Winyard, PJ;Long, DA;2016Loss of endogenous thymosin β4 accelerates glomerular disease. Kidney Int. PubMed PMID: 27575556
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
Purification:
Immunoaffinity Chromatography
Buffer System:
PBS, pH 7.6
Preservatives:
0.09% Sodium Azide
Stabilizers:
1% BSA
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (expected):
Dog, Mouse, Rat, Pig, Rabbit.
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody recognizes Wilm's Tumor Protein.
Cellular Localization: Nuclear.
Gene ID 7490

Accessory Products

Proteins and/or Positive Controls

Proteins for WT1 / Wilms tumor protein (7 products)

Catalog No. Species Pres. Purity   Source  

WT1 / Wilms tumor protein (full length, N-term HIS tag, transcript variant B)

WT1 / Wilms tumor protein Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

WT1 / Wilms tumor protein (full length, N-term HIS tag, transcript variant A)

WT1 / Wilms tumor protein Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

WT1 / Wilms tumor protein

WT1 / Wilms tumor protein Human
  Abnova Taiwan Corp.

WT1 / Wilms tumor protein

WT1 / Wilms tumor protein Human Purified
  Abnova Taiwan Corp.

WT1 / Wilms tumor protein

WT1 / Wilms tumor protein Human Purified
  Abnova Taiwan Corp.

WT1 / Wilms tumor protein

WT1 / Wilms tumor protein Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

WT1 / Wilms tumor protein

WT1 / Wilms tumor protein Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Positive controls for WT1 / Wilms tumor protein (1 products)

Catalog No. Species Pres. Purity   Source  

Wilms Tumor 1 Lysate

Western Blot: Wilms Tumor 1 Lysate [NBL1-17887] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for WT1
  Novus Biologicals Inc.
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