discontinued

ARP37988_P050 WT1 / Wilms tumor protein antibody

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Rabbit anti Human WT1 / Wilms tumor protein

Product Description for WT1 / Wilms tumor protein

Rabbit anti Human WT1 / Wilms tumor protein.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for WT1 / Wilms tumor protein

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms WT33
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Europe only
PDF datasheet View Datasheet
Manufacturer AVIVA Systems Biology

Datasheet Extract

Immunogen
Swiss Prot Num:
P19544
Immunogen:
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human WT1 with an internal ID of P20090
GeneID:
7490
Add. information Rabbit Ig G is purified by peptide affinity chromatography method. 56kDa
Application Suggested dilution at 1 ELISA titre using peptide based assay: 1:312500
Background WT1 is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm\'s tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms.This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm\'s tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.
General Readings Ruteshouser,E.C., (2008) Genes Chromosomes Cancer 47 (6), 461-470
Storage Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 ul of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20DegC. Avoid repeat freeze-thaw cycles.
Format
Purification:
Rabbit Ig G is purified by peptide affinity chromatography method.
Buffer System:
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 ul of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20DegC. Avoid repeat freeze-thaw cycles.
State:
Aff - Purified
Specificity
Species:
Human
Gene ID 7490

Accessory Products

Proteins and/or Positive Controls

Proteins for WT1 / Wilms tumor protein (7 products)

Catalog No. Species Pres. Purity   Source  

WT1 / Wilms tumor protein (full length, N-term HIS tag, transcript variant B)

WT1 / Wilms tumor protein Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

WT1 / Wilms tumor protein (full length, N-term HIS tag, transcript variant A)

WT1 / Wilms tumor protein Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

WT1 / Wilms tumor protein

WT1 / Wilms tumor protein Human
  Abnova Taiwan Corp.

WT1 / Wilms tumor protein

WT1 / Wilms tumor protein Human Purified
  Abnova Taiwan Corp.

WT1 / Wilms tumor protein

WT1 / Wilms tumor protein Human Purified
  Abnova Taiwan Corp.

WT1 / Wilms tumor protein

WT1 / Wilms tumor protein Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

WT1 / Wilms tumor protein

WT1 / Wilms tumor protein Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Positive controls for WT1 / Wilms tumor protein (1 products)

Catalog No. Species Pres. Purity   Source  

Wilms Tumor 1 Lysate

Western Blot: Wilms Tumor 1 Lysate [NBL1-17887] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for WT1
  Novus Biologicals Inc.
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