Secondary Antibodies

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Secondary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Human IgG

Human IgG Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt WB
0.8 mg / €300.00
  SICGEN

Human IgG (H+L chain), Fab Fragment

Western Blot of Goat anti-Fab Human IgG (H&L) Antibody Fluorescein Conjugated: 
Lane 1: Human IgG 
Load: 50 ng per lane 
Secondary antibody: Fab Human IgG (H&L) Antibody Fluorescein Conjugated at 1/1,000 for 60 min at RT Block: MB-070 for 30 min at RT 
Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa Goat FITC
1 mg / €220.00
  OriGene Technologies GmbH

Rabbit IgG (H+L chain)

Western Blot of Peroxidase Conjugated Goat Anti-Rabbit Antibody: 
Lane 1: HeLa Whole Cell Lysate. 
Lane 2: NIH/3T3 Whole Cell Lysate. 
Load: 10 µg per lane. 
Primary antibody: Beta Actin Antibody at 1/1000 for overnight at 4°C. 
Secondary antibody: Peroxidase Conjugated Goat Anti-Rabbit Antibody at 1:40,000 for 30 min at RT. 
Block: MB-070 for 30 min at RT .
Predicted/Observed size: 42 kDa. Goat HRP Rb E, P, WB
2 mg / €220.00
  OriGene Technologies GmbH

Rabbit IgG (H+L chain, adsorbed)

Shown here is a serial 1/1 dilution of Control Rabbit IgG protein (Cat.-No RA032, 250ng starting total load) co incubated with HRP conjugated Goat anti Rabbit IgG Cat.-No R1454HRP and Dylight 649 conjugated Goat anti Rabbit IgG  1/20K in MB-070. Blot was dried and imaged (A) on Biorad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager. Goat HRP E, WB
1 mg / €330.00
  OriGene Technologies GmbH

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