Secondary Antibodies

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Secondary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Chicken IgM (Mu heavy chain specific)

Immunofluorescent Staining: Chicken peripheral blood mononuclear cells were double-stained with Mouse anti-Chicken IgM-FITC and Mouse anti-Chicken CD3-PE. Small lymphocytes were then gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 µg/10e6 cells. Mouse IgG2b M-1 FITC Chk F
0.5 mg / €450.00
  Acris Antibodies GmbH

Hamster IgG (adsorbed)

Detection of Hamster anti Human EMR1 (clone A10) in a Flow Cytometric analysis of Human eosinophils using Goat anti Hamster IgG -Biotin (Cat.-No SP1025B) visualised by Streptavidin -RPE Goat IgG Biotin Hst E, F
0.8 mg / €700.00
  Acris Antibodies GmbH

Human IgE

Fig. 1. Flow Cytometry analysis of basophil activation upon stimulation of normal (heparin-treated) whole blood with combination of IL-3 and Goat anti-IgE polyclonal antibody.
Combination of anti-human IgE (BE5) FITC and anti-human CD63 (MEM-259) PE-Dyomics 647 was used (analysis in basophil window). 
Fig. 1A - staining of non-stimulated (control) sample
Fig. 1B - staining of IgE stimulated sample Mouse IgG1 BE5 FITC Hu F
0.1 mg / €230.00
  Acris Antibodies GmbH

Human IgE

Figure 2: staining of IgE stimulated sample Mouse IgG1 BE5 Purified Hu E, F
0.1 mg / €180.00
  Acris Antibodies GmbH

Human IgE

Fig. 1. Flow Cytometry analysis of basophil activation upon stimulation of normal (heparin-treated) whole blood with combination of IL-3 and Goat anti-IgE polyclonal antibody.
Combination of anti-human IgE (BE5) FITC and anti-human CD63 (MEM-259) PE-Dyomics 647 was used (analysis in basophil window). 
Fig. 1A - staining of non-stimulated (control) sample
Fig. 1B - staining of IgE stimulated sample Mouse IgG1 BE5 PE Hu F
0.1 mg / €270.00
  Acris Antibodies GmbH

Human IgG (F(ab)2 specific)

Western Blotting analysis (reducing conditions) of human IgG Fab fragment using anti-human IgG Fab fragment (4A11). Lane 1: Normal human immunoglobulins. Lane 2: Normal bovine immunoglobulins. Mouse IgG2b 4A11 Purified Hu E, F, WB
0.1 mg / €180.00
  Acris Antibodies GmbH

Human IgG (F(ab)2 specific)

Surface staining of human peripheral blood cells with anti-human IgG Fab fragment (4A11) PE. Mouse IgG2b 4A11 PE Hu F
0.1 mg / €270.00
  Acris Antibodies GmbH

Human IgM (Fc-region)

Surface staining of Human peripheral blood cells with anti-Human IgM Antibody (CH2) APC conjuagetd Cat.-No SM3063APC. Cells in the lymphocyte gate were used for analysis. Mouse IgG1 CH2 APC Hu F
0.1 mg / €270.00
  Acris Antibodies GmbH

Human IgM (Fc-region)

Surface staining of human peripheral blood cells with anti-human IgM (CH2) PE. Mouse IgG1 CH2 FITC Hu F
0.1 mg / €230.00
  Acris Antibodies GmbH

Human IgM (Fc specific)

Surface staining of human peripheral blood cells with anti-human IgM (CH2) APC.
Cells in the lymphocyte gate were used for analysis. Mouse IgG1 CH2 HRP Hu WB
0.1 mg / €270.00
  Acris Antibodies GmbH

Human IgM (Fc-region)

Surface staining of human peripheral blood cells with anti-human IgM (CH2) APC. Cells in the lymphocyte gate were used for analysis. Mouse IgG1 CH2 Purified Hu E, F, ICC/IF, WB
0.1 mg / €180.00
  Acris Antibodies GmbH

Human IgM (Fc specific)

Surface staining of human peripheral blood cells with anti-human IgM (CH2) APC.
Cells in the lymphocyte gate were used for analysis. Mouse IgG1 CH2 PE Hu F
0.1 mg / €270.00
  Acris Antibodies GmbH

Mouse IgA+IgG+IgM (H+L chain)

Immunofluorescent Staining: Human peripheral blood mononuclear cells were labeled with either Mouse anti-Human CD3 (IgG1) (A), anti-Human CD5 (IgG2a) (B), anti-Human CD22 (IgG2b) (C), anti-Human IgD (IgG3) (D) or anti-Human CD57 (IgM) (E). After washing the cells were then stained with Goat anti-Mouse Ig-R-PE Cat.-No AP32278RP (0.1 µg/106 cells), following which lymphocytes were gated and analyzed by Flow Cytometry. Goat IgG PE Ms C, E, F, ICC/IF
0.5 mg / €380.00
  Acris Antibodies GmbH

Mouse IgA+IgG+IgM (H+L chain)

Immunofluorescent Staining: Human peripheral blood mononuclear cells were labeled with either Mouse anti-Human CD3 (IgG1) (A), anti-Human CD5 (IgG2a) (B), anti-Human CD22 (IgG2b) (C), anti-Human IgD (IgG3) (D) or anti-Human CD57 (IgM) (E). After washing the cells were then stained with Goat anti-Mouse Ig-R-PE Cat.-No AP32278RP (0.1 µg/106 cells), following which lymphocytes were gated and analyzed by Flow Cytometry. Goat IgG PE Ms C, E, F, ICC/IF
0.25 mg / €260.00
  Acris Antibodies GmbH

Mouse IgD (Delta heavy chain)

BALB/c splenocytes were double-stained with rat anti mouse IgD-FITC and rat anti-mouse CD19-R-PE Small lymphocytes were then gated and analyzed using a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Rat IgG2a 11-26 FITC Ms F, ICC/IF
0.5 mg / €320.00
  Acris Antibodies GmbH

Mouse IgG (H+L chain), adsorbed


Detection of Mouse anti Rat RT1Ac Antibody in a Flow Cytometric analysis of BN Rat spleen cells using Goat anti Mouse IgG Antibody -FITC Cat.-No SP1041F
Goat FITC Ms F
0.5 mg / €330.00
  Acris Antibodies GmbH

Mouse IgG (adsorbed)

Rabbit peripheral blood granulocytes stained with Mouse anti Rabbit CD44 (Cat.-No SM607) followed by Goat anti Mouse IgG:FITC (Cat.-No AP05383FC-N) Goat IgG FITC Ms F
0.5 mg / €330.00
  Acris Antibodies GmbH

Mouse IgG2a (gamma 2a chain specific)

Immunofluorescent Staining: Human PBMC were incubated with either mouse IgG1 anti-CD3, mouse IgG2a anti-CD5, mouse IgG2b anti-
CD22, or mouse IgG3 anti-IgD. After washing, the cells were then incubated with FITC-labeled rat anti-mouse IgG1. Small lymphocytes were gated and analyzed on a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 1 µg/10e6 cells. Rat IgG1 SB84a FITC Ms F, ICC/IF
0.5 mg / €280.00
  Acris Antibodies GmbH

Mouse IgM (Mu chain specific)

Immunofluorescent Staining: Cells from BALB/c spleen were double-stained with rat anti-Mouse IgM-PE (Cat#AM08015RP-N or AM08015RP-S) and Rat anti-Mouse CD19-FITC (Clone 6D5), following which small lymphocytes were gated and analyzed using a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 0.3 µg/10e6 cells. Rat IgG1 1B4B1 PE Ms F, ICC/IF
0.2 mg / €410.00
  Acris Antibodies GmbH

Mouse IgM (Mu chain specific)

Immunofluorescent Staining: Cells from BALB/c spleen were double-stained with rat anti-Mouse IgM-PE (Cat#AM08015RP-N or AM08015RP-S) and Rat anti-Mouse CD19-FITC (Clone 6D5), following which small lymphocytes were gated and analyzed using a FACScan(TM) flow cytometer (BDIS, San Jose, CA). Amount Used: 0.3 µg/10e6 cells. Rat IgG1 1B4B1 PE Ms F, ICC/IF
0.1 mg / €310.00
  Acris Antibodies GmbH

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