Product Description for Bovine IgG
Product is tested for Immunodiffusion, Immunoprecipitation.
Properties for Bovine IgG
|Product Category||Secondary Antibodies|
|Synonyms||Bovine Immunoglobulin G|
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Material safety datasheet||MSDS for Polyclonal Antibodies (de)|
|Add. information||Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.|
|Application||Can be used in precipitating techniques as Immunoelectrophoresis and Radial Immunodiffusion to identify the presence of IgG in bovine serum or other body fluids or to determine its concentration.
To prepare an Immunoadsorbent for the purification of Bovine IgG from serum or plasma.
Immunoelectrophoresis: Use 2 µl serum or equivalent against 120 µl antiserum.
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
Precipitin titre: 1/64 when tested against pooled normal bovine serum in agar-block Immunodiffusion titration.
|Concentration||Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.|
|Storage||Lyophilized antiseum can be shipped at ambient temperature and may be stored at 2-8°C or at -20°C for prolonged storage.
Dilutions may be prepared by adding PBS, pH 7.2.
If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum.
Diluted antiserum should be stored at 2-8°C, not refrozen, and preferably used the same day.
Avoid Repeated thawing and freezing.
Shelf life: one year from despatch.