R1304AP Bovine IgG (H+L chain) antibody

Search for all "Bovine IgG"

1 mg / €270.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.


Product Description for Bovine IgG

Properties: (H+L chain)
Presentation: AP
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Bovine IgG

Product Category Secondary Antibodies
Target Category
Quantity 1 mg
Synonyms Bovine Immunoglobulin G
Presentation AP
Reactivity Bov
Applications C, E, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Bovine IgG whole molecule.
Application Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for this product.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
General Readings Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971. (Conjugation)
Storage Store the antibody undiluted at 2-8°C.
Freezing alkaline phosphatase conjugates will result in a substantial loss
of enzymatic activity.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Immunoaffinity chromatography.
Buffer System:
0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 0.01% sodium azide as preservative and 10 mg/ml Polyethylene Glycol (PEG-8000) as stabilizer.
Liquid (sterile filtered) purified Ig fraction.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Bovine and Bovine Serum.
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