R1304B Bovine IgG (H+L chain) antibody

Search for all "Bovine IgG"

2 mg / €230.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1304B

Product Description for Bovine IgG

Properties: (H+L chain)
Presentation: Biotin
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Bovine IgG

Product Category Secondary Antibodies
Target Category
Quantity 2 mg
Synonyms Bovine Immunoglobulin G
Presentation Biotin
Reactivity Bov
Applications C, E, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Bovine IgG whole molecule
Application Immunoblotting (1/2,000-1/10,000).
ELISA (1/20,000-1/100,000).
Immunohistochemistry (1/1,000-1/5,000).
Immunomicroscopy.
as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. 
This product has been assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000-1/20,000 of the reconstitution concentration is suggested for this product.
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
General Readings Conjugation Reference: Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin
Molar radio:
10-20 BAC molecules per Goat IgG molecule.
Purification:
Immunoaffinity Chromatography
Buffer System:
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
Preservatives:
0.01% (w/v) Sodium Azide
Stabilizers:
10 mg/ml Polyethylene Glycol (PEG-8000)
State:
Lyophilized purified IgG fraction
Reconstitution:
Restore with deionized water (or equivalent)
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Bovine IgG and Bovine Serum.
Species:
Bovine.
  • LinkedIn