R1304F Bovine IgG (H+L chain) antibody

Search for all "Bovine IgG"

2 mg / €220.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1304F

Product Description for Bovine IgG

Properties: (H+L chain)
Presentation: FITC
Product is tested for Immunocytochemistry/Immunofluorescence.

Properties for Bovine IgG

Product Category Secondary Antibodies
Target Category
Quantity 2 mg
Synonyms Bovine Immunoglobulin G
Presentation FITC
Reactivity Bov
Applications ICC/IF
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Bovine IgG, whole molecule
Application Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Concentration 2.0 mg/ml (by UV absorbance at 280 nm)
General Readings Blakeslee & Baines, J. Immunol. Methods 13; 305-320, 1976. (Conjugation)
Storage Store vial at 2-8°C prior to restoration.
Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
Format
Label:
Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) which has identical fluorescence properties as
FITC
Absorption emission:
495 nm / 528 nm
Molar radio:
4.2 moles DTAF per mole of Rabbit IgG
Purification:
Immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorptions to remove any unwanted reactivities
Buffer System:
0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4, with 0.01% (w/v) Thimerisol as preservative and 10 mg/ml Polyethylene Glycol (PEG mw 8,000) as stabilizer
State:
Lyophilized purified IgG fraction
Reconstitution:
Restore with 1.0 ml of deionized water or equivalent.
Specificity
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Bovine IgG, Bovine IgG and Bovine Serum.
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