Product Description for Bovine IgG
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.
Properties for Bovine IgG
|Product Category||Secondary Antibodies|
|Synonyms||Bovine Immunoglobulin G|
|Applications||C, E, ICC/IF, WB|
|PDF datasheet||View Datasheet|
|Manufacturer||Acris Antibodies GmbH|
|Material safety datasheet||MSDS for Polyclonal Antibodies (de)|
|Add. information||Do Not Add Use Sodium Azide.|
|Application||Suitable for Immunoblotting (Western blot: 1/1,000-1/10,000 or Dot blot), ELISA (1/10,000-1/50,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
|Concentration||2.0 mg/ml (by UV absorbance at 280 nm)|
|General Readings||Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation)|
|Storage||Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.