R1305AP Bovine IgG (Fc specific) antibody

Search for all "Bovine IgG"

1 mg / €300.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1305AP

Product Description for Bovine IgG

Properties: (Fc specific)
Presentation: AP
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Bovine IgG

Product Category Secondary Antibodies
Target Category
Quantity 1 mg
Synonyms Bovine Immunoglobulin G
Presentation AP
Reactivity Bov
Applications C, E, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Bovine IgG F(c) fragment.
Application Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product.
Concentration 0.41 mg/ml (by UV absorbance at 280 nm)
General Readings Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971. (Conjugation)
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
Freezing alkaline phosphatase conjugates will result in a substantial loss
of enzymatic activity.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
Format
Label:
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
AP
Purification:
Immunoaffinity chromatography.
Buffer System:
0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative.
State:
Liquid (sterile filtered) purified Ig fraction.
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Bovine IgG and Bovine Serum.
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