R1305F Bovine IgG (Fc specific) antibody

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1.5 mg / €230.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1305F

Product Description for Bovine IgG

Properties: (Fc specific)
Presentation: FITC
Product is tested for Immunocytochemistry/Immunofluorescence.

Properties for Bovine IgG

Product Category Secondary Antibodies
Target Category
Quantity 1.5 mg
Synonyms Bovine Immunoglobulin G
Presentation FITC
Reactivity Bov
Applications ICC/IF
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Bovine IgG F(c) fragment.
Application Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Concentration 1.5 mg/ml (by UV absorbance at 280 nm)
General Readings The and Feltkamp, Immunology 18; 865, 1970. (Conjugation)
Storage Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Label:
Fluorescein isothiocyanate (Molecular Weight 390 daltons)
FITC
Absorption emission:
495 nm / 528 nm
Molar radio:
3.6 moles FITC per mole of Rabbit IgG.
Purification:
Immunoaffinity chromatography.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative.
State:
Lyophilized purified Ig fraction.
Reconstitution:
Restore with 1.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- Fluorescein, anti-Rabbit Serum, Bovine IgG, Bovine IgG F(c) and Bovine Serum.
No reaction was observed against Bovine IgG F(ab')2.
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