R1305HRP Bovine IgG (Fc specific) antibody

Search for all "Bovine IgG"

1.5 mg / €290.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

R1305HRP

Product Description for Bovine IgG

Properties: (Fc specific)
Presentation: HRP
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Bovine IgG

Product Category Secondary Antibodies
Target Category
Quantity 1.5 mg
Synonyms Bovine Immunoglobulin G
Presentation HRP
Reactivity Bov
Applications C, E, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Bovine IgG F(c) fragment
Application Suitable for immunoblotting (western or dot blot 1:1,000 - 1:10,000), ELISA (1:10,000 - 1:50,000=, immunoperoxidase electron microscopy and immunohistochemistry (1:500 - 1;2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.
Concentration 1.5 mg/ml (by UV absorbance at 280 nm)
General Readings (Conjugation) Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
Storage Store vial at 2-8°C prior to restoration.
Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
Format
Label:
Horseradish Peroxidase
HRP
Purification:
Immunoaffinity chromatography
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Polyethylene Glycol (PEG-8000) as a stabilizer and 0.01% (w/v) Gentamicin Sulfate as a preservative
State:
Lyophilized Ig fraction
Reconstitution:
Restore with 1.0 ml of deionized water or equivalent.
Specificity
Specificity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Bovine IgG, Bovine IgG F(c) and Bovine Serum.
No reaction was observed against Bovine IgG F(ab')2.
  • LinkedIn