SM1499P Canine IgE antibody

Search for all "Canine IgE"

0.25 mg / €520.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

SM1499P

Product Description for Canine IgE

Presentation: Purified
Product is tested for Western blot / Immunoblot, Enzyme Immunoassay.

Properties for Canine IgE

Product Category Secondary Antibodies
Quantity 0.25 mg
Synonyms Dog IgE, Dog Immunoglobulin E
Presentation Purified
Reactivity Can
Applications E, WB
Clonality Monoclonal
Clone E6-71A1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Affinity purified IgE preparation from heavily parasitized and allergic dog serum.
Isotype control AM03095PU-N
Application ELISA.
Western Blotting: Under reducing conditions, this antibody detects a single major band of approximately 62-65 kDa in samples of affinity purified Dog IgE.
Background IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies -and the life threatening respiratory distress of asthma and anaphylactic shock.
Concentration 1.0 mg/ml
General Readings
  • Ognjenovic J, Milcic-Matic N, Smiljanic K, Vuckovic O, Burazer L, Popovic N, et al. Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis. Vet Immunol Immunopathol. 2013 Sep 1;155(1-2):38-47. doi: 10.1016/j.vetimm.2013.06.005. Epub 2013 Jun 7. PubMed PMID: 23830203.
  • Martins, L.M. et al. (2017) Allergy to grass pollen: mapping of Dactylis glomerata and Phleum pratense allergens for dogs by two-dimensional immunoblotting. Postepy Dermatol Alergol. 34 (1): 60-9. PubMed PMID: 28261033
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A from tissue culture supernatant
Buffer System:
PBS, pH 7.2
Preservatives:
0.05% Sodium Azide
State:
Liquid purfied IgG fraction
Purified
Specificity
Specificity:
This Mouse anti Dog IgE antibody, clone E6-71A1, recognizes Canine IgE. It does not cross react with Dog IgM, IgA, IgG1 or IgG2.
Western blot analysis against affinity purified dog IgE using Mouse anti Dog IgE clone E6-71A1 demonstrates a single major band of 62-65kDa under reducing conditions.
Species:
Canine (Dog)
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